High-yield bioactive triterpenoid production by heterologous expression in Nicotiana benthamiana using the Tsukuba system

J Romsuk, S Yasumoto, EO Fukushima… - Frontiers in Plant …, 2022 - frontiersin.org
Oleanolic acid is a pentacyclic triterpenoid found in numerous plant species and is a
precursor to several bioactive triterpenoids with commercial potential. However, oleanolic …

Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus

P Istiandari, S Yasumoto, H Seki… - Frontiers in Plant …, 2023 - frontiersin.org
Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the
structural diversification of triterpenoids. To perform site-specific oxidations of the triterpene …

Cloning and Functional Characterization of NADPH-Cytochrome P450 Reductases in Aconitum vilmorinianum

J Cheng, G Li, X Wang, C Yang, F Xu, Z Qian, X Ma - Molecules, 2023 - mdpi.com
Diterpenoid alkaloids (DAs) are major pharmacologically active ingredients of Aconitum
vilmorinianum, an important medicinal plant. Cytochrome P450 monooxygenases (P450s) …

Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltrated Nicotiana benthamiana leaves

J Romsuk, P Srisawat, J Robertlee, S Yasumoto… - Plant …, 2024 - jstage.jst.go.jp
Triterpenoids, a group of specialized plant metabolites with substantial structural diversity,
are promising for healthcare applications. Ursolic acid, a pentacyclic triterpenoid with …

[HTML][HTML] Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast

S Qiu, MDS Gilani, C Müller, RM Zarazua-Navarro… - New …, 2024 - Elsevier
Ginseng, a cornerstone of traditional herbal medicine in Asia, garnered significant attention
for its therapeutic potential. Central to its pharmacological effects are ginsenosides, the …

Study on Revealing Peanut-Related Disease Prevention Gene Clusters via Whole Transcriptome Sequencing

H Du, N Lu, C Li - Agriculture, 2023 - mdpi.com
Root rot caused by Fusarium oxysporum is a serious root disease that affects the growth and
yield of peanut crops worldwide. Identifying resistance genes is of great significance for both …

Heterologous expression in E. coli and functional characterization of the tomato CPR enzymes

W Choi, SY Park, HM Kim, TD Mai, JH Do… - Applied Biological …, 2023 - Springer
Abstract NADPH-cytochrome P450 reductase (CPR) is a key enzyme transferring electrons
to cytochrome P450. In tomatoes (Solanum lycopersicum), two CPR genes, SlCPR1 and …

Unraveling the role of P450 reductase in herbicide metabolic resistance mechanism

CAG Rigon, S Iwakami, TA Gaines, FE Dayan - BioRxiv, 2023 - biorxiv.org
Plants require cytochrome P450 reductase (CPR) to supply two electrons for cytochrome
P450 monooxygenase enzymes (P450) to react with an organic substrate. The transfer of …

Bioprocess optimization enables enhanced protopanaxadiol production in yeast

S Qiu, MDS Gilani, C Müller, U Liebal, LM Blank - bioRxiv, 2023 - biorxiv.org
Ginsenosides are the primary active metabolites of ginseng and have been used in
traditional herbal medicine in Asia for more than 4,000 years. Protopanaxadiol (PPD) is the …

Las reductasas NADPH-citocromo P450 de clase I y II exhiben diferentes funciones en la biosíntesis de triterpenoides en Lotus japonicus

P Istiandari, S Yasumoto, H Seki, EO Fukushima - 2023 - lareferencia.info
Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the
structural diversification of triterpenoids. To perform site-specific oxidations of the triterpene …