Group I introns fold to form an active site to mediate their own RNA splicing. Sequence
elements conserved among the available set of 66 group I introns are compiled …

Certain RNA molecules can mediate their own cleavage or splicing or act as enzymes to
promote reactions on substrate RNA molecules. Thus, RNA is not restricted to being a …

THE discovery of RNA enzymes1, 2 has, for the first time, provided a single molecule that
has both genetic and catalytic properties. We have devised techniques for the mutation …

The telomerase enzyme of Tetrahymena synthesizes repeats of the telomeric DNA
sequence TTGGGG de novo in the absence of added template. The essential RNA …

Five small nuclear RNAs (U1, U2, U4, U5, and U6) participate in precursor messenger RNA
(pre-mRNA) splicing. To probe their interactions within the active center of the mammalian …

Proteins are not the only catalysts of cellular reactions; there is a growing list of RNA
molecules that catalyze RNA cleavage and joining reactions. The chemical mechanisms of …

Ribozymes are RNA molecules that catalyze biochemical reactions. Fe (II)-EDTA, a solvent-
based reagent which cleaves both double-and single-stranded RNA, was used to …

G2CCCUCU+ GA5 (G= guanosine). This reaction is analogous to the first step in self-
splicing of the pre-rRNA, with the product G2CCCUCU analogous to the 5'-exon. The …

The higher order folding process of the catalytic RNA derived from the self-splicing intron of
Tetrahymena thermophila was monitored with the use of Fe (II)-EDTA-induced free radical …

RIBOZYMES can be targeted to cleave specific RNAs1–8, which has led to much interest in
their potential as gene inhibitors3, 9, 10. Such fra/is-cleaving ribozymes join a growing list of …