[HTML][HTML] The safety and anti-ageing effects of nicotinamide mononucleotide in human clinical trials: an update

Q Song, X Zhou, K Xu, S Liu, X Zhu, J Yang - Advances in nutrition, 2023 - Elsevier
The importance of nicotinamide adenine dinucleotide (NAD+) in human physiology is well
recognized. As the NAD+ concentration in human skin, blood, liver, muscle and brain are …

[HTML][HTML] Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration

JR Nielsen, RA Weusthuis, WE Huang - Biotechnology Advances, 2023 - Elsevier
Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …

Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide

Z Huang, N Li, S Yu, W Zhang, T Zhang… - ACS Synthetic …, 2022 - ACS Publications
Research studies on NAD+ have proven its crucial role in aging and disease. Nicotinamide
mononucleotide (NMN), as the key intermediate of NAD+, plays a significant role in …

Metabolic design for selective production of nicotinamide mononucleotide from glucose and nicotinamide

S Shoji, T Yamaji, H Makino, J Ishii, A Kondo - Metabolic Engineering, 2021 - Elsevier
Abstract β-Nicotinamide mononucleotide (NMN) is, one of the nucleotide compounds, a
precursor of NAD+ and has recently attracted attention as a nutraceutical. Here, we develop …

Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

L Zhang, E King, WB Black, CM Heckmann… - Nature …, 2022 - nature.com
Noncanonical redox cofactors are attractive low-cost alternatives to nicotinamide adenine
dinucleotide (phosphate)(NAD (P)+) in biotransformation. However, engineering enzymes to …

Streamlining design, engineering, and applications of nicotinamide riboside kinase for sustainable biosynthesis of nicotinamide mononucleotide in flow

F Cheng, XH Wu, H Li, KX Li, Q Guo… - ACS Sustainable …, 2023 - ACS Publications
The market demand for β-nicotinamide mononucleotide (NMN), an antiaging health product,
is rapidly expanding. Consequently, there is an urgent requirement for an environment …

Metabolic engineering of Escherichia coli for biosynthesis of β‐nicotinamide mononucleotide from nicotinamide

Y Liu, M Yasawong, B Yu - Microbial Biotechnology, 2021 - Wiley Online Library
The β‐nicotinamide mononucleotide (NMN) is a key intermediate of an essential coenzyme
for cellular redox reactions, NAD. Administration of NMN is reported to improve various …

Biosynthesis of a therapeutically important nicotinamide mononucleotide through a phosphoribosyl pyrophosphate synthetase 1 and 2 engineered strain of …

A Maharjan, M Singhvi, BS Kim - ACS Synthetic Biology, 2021 - ACS Publications
Nicotinamide mononucleotide (NMN), a precursor of NAD+, can be synthesized by the
conversion of nicotinamide with the help of nicotinamide phosphoribosyl transferase …

A holistic approach for process intensification of nicotinamide mononucleotide production via high cell density cultivation under exponential feeding strategy

SR Kafle, A Kushwaha, L Goswami, A Maharjan… - Bioresource …, 2023 - Elsevier
Nicotinamide mononucleotide (NMN) subsists in all living organisms and has drawn
tremendous attention as a nutraceutical and pharmaceutical product for several diseases …

Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

E King, S Maxel, Y Zhang, KC Kenney, Y Cui… - Nature …, 2022 - nature.com
Noncanonical cofactor biomimetics (NCBs) such as nicotinamide mononucleotide (NMN+)
provide enhanced scalability for biomanufacturing. However, engineering enzymes to …