Protein splicing of inteins: a powerful tool in synthetic biology

H Wang, L Wang, B Zhong, Z Dai - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Inteins are protein segments that are capable of enabling the ligation of flanking extein into a
new protein, a process known as protein splicing. Since its discovery, inteins have become …

Recent advances in molecular farming using monocot plants

M Mirzaee, Z Osmani, J Frébortová, I Frébort - Biotechnology Advances, 2022 - Elsevier
Heterologous synthesis of proteins or peptides in plant-based systems, referred to as plant
molecular farming, is a practical and safe approach for the large-scale and cost-effective …

KCNQ2 and KCNQ5 form heteromeric channels independent of KCNQ3

H Soh, K Springer, K Doci… - Proceedings of the …, 2022 - National Acad Sciences
KCNQ2 and KCNQ3 channels are associated with multiple neurodevelopmental disorders
and are also therapeutic targets for neurological and neuropsychiatric diseases. For more …

Inteins as drug targets and therapeutic tools

AM Tharappel, Z Li, H Li - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Multidrug-resistant pathogens are of significant concern in recent years. Hence new
antifungal and anti-bacterial drug targets are urgently needed before the situation goes …

Molecular design of protein-based materials–state of the art, opportunities and challenges at the interface between materials engineering and synthetic biology

E Shire, AAB Coimbra, CB Ostria… - … Systems Design & …, 2024 - pubs.rsc.org
Structural proteins like silk, squid ring teeth, elastin, collagen, or resilin, among others, are
inspiring the development of new sustainable biopolymeric materials for applications …

Inteins: A Swiss army knife for synthetic biology

S Anastassov, M Filo, M Khammash - Biotechnology Advances, 2024 - Elsevier
Inteins are proteins found in nature that execute protein splicing. Among them, split inteins
stand out for their versatility and adaptability, presenting creative solutions for addressing …

Increasing the Scalability of Toxin–Intein Orthogonal Combinations

R López-Igual, P Dorado-Morales… - ACS Synthetic …, 2023 - ACS Publications
Inteins are proteins embedded into host proteins from which they are excised in an
autocatalytic reaction. Specifically, split inteins are separated into two independent …

Protein engineering for natural product biosynthesis: expanding diversity for therapeutic applications

SO Otun, JA Lerma-Escalera, K Ntushelo… - Journal of Bio-X …, 2023 - spj.science.org
In this dispensation of the fourth industrial revolution, protein engineering has become a
popular approach for increasing enzymatic activity, stability, and titer in the biosynthesis of …

Intein inhibitors as novel antimicrobials: protein splicing in human pathogens, screening methods, and off-target considerations

DA Wall, SP Tarrant, C Wang, KV Mills… - Frontiers in Molecular …, 2021 - frontiersin.org
Protein splicing is a post-translational process by which an intervening polypeptide, or
intein, catalyzes its own removal from the flanking polypeptides, or exteins, concomitant with …

Calcimycin Inhibits Cryptococcus neoformans In Vitro and In Vivo by Targeting the Prp8 Intein Splicing

AM Tharappel, Z Li, YC Zhu, X Wu… - ACS infectious …, 2022 - ACS Publications
Drug resistance is a significant concern in the treatment of diseases, including
cryptococcosis caused by Cryptococcus neoformans (Cne) and Cryptococcus gattii (Cga) …