Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

[HTML][HTML] Single-photon avalanche diode imagers in biophotonics: review and outlook

C Bruschini, H Homulle, IM Antolovic, S Burri… - Light: Science & …, 2019 - nature.com
Single-photon avalanche diode (SPAD) arrays are solid-state detectors that offer imaging
capabilities at the level of individual photons, with unparalleled photon counting and time …

Single-molecule fluorescence lifetime imaging using wide-field and confocal-laser scanning microscopy: A comparative analysis

N Oleksiievets, C Mathew, JC Thiele, JI Gallea… - Nano Letters, 2022 - ACS Publications
A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-
lifetime single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and …

Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …

Wide-field time-gated SPAD imager for phasor-based FLIM applications

A Ulku, A Ardelean, M Antolovic, S Weiss… - … and applications in …, 2020 - iopscience.iop.org
We describe the performance of a new wide area time-gated single-photon avalanche diode
(SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal …

[HTML][HTML] Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

V Zickus, ML Wu, K Morimoto, V Kapitany, A Fatima… - Scientific Reports, 2020 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct
insight into cell metabolism, cell dynamics and protein activity. However, determining the …

[HTML][HTML] Fast timing techniques in FLIM applications

LM Hirvonen, K Suhling - Frontiers in Physics, 2020 - frontiersin.org
Fluorescence lifetime imaging (FLIM) is increasingly used in many scientific disciplines,
including biological and medical research, materials science and chemistry. The …

[HTML][HTML] Single photon avalanche diode arrays for time-resolved Raman spectroscopy

F Madonini, F Villa - Sensors, 2021 - mdpi.com
The detection of peaks shifts in Raman spectroscopy enables a fingerprint reconstruction to
discriminate among molecules with neither labelling nor sample preparation. Time-resolved …

[HTML][HTML] Photophysical properties and photonic applications of porphyrin-based MOFs

SM Sajjadinezhad, L Boivin, K Bouarab… - Coordination Chemistry …, 2024 - Elsevier
Metal-organic frameworks (MOFs) are a notorious class of porous materials that gained
significant attention in recent years due to their unique structural and rich functional …

[HTML][HTML] High-throughput, multi-parametric, and correlative fluorescence lifetime imaging

C Poudel, I Mela, CF Kaminski - Methods and Applications in …, 2020 - iopscience.iop.org
In this review, we discuss methods and advancements in fluorescence lifetime imaging
microscopy that permit measurements to be performed at faster speed and higher resolution …