Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Functionalized magnetic materials (FMMs) have been widely used in analytical chemistry.
For sample preparation, FMMs show many advantages including easy surface modification …

Rapid changes of protein phosphorylation play a crucial role in the regulation of many
cellular processes. Being post-translationally modified, phosphoproteins are often present in …

The elucidation of protein post-translational modifications, such as phosphorylation, remains
a challenging analytical task for proteomic studies. Since many of the proteins targeted for …

Mass spectrometry is the tool of choice to investigate protein phosphorylation, which plays a
vital role in cell regulation and diseases such as cancer. However, low abundances of …

Large scale characterization of phosphoproteins requires highly specific methods for
purification of phosphopeptides because of the low abundance of phosphoproteins and …

The demand for effective sample preparation strategies that enable the in-depth study of
protein phosphorylation has spawned a number of selective affinity techniques that allow …

Sample preparation has been playing an important role in the analysis of complex samples.
Mesoporous materials as the promising adsorbents have gained increasing research …

The mixture of phosphopeptides enriched from proteome samples are very complex. To
reduce the complexity it is necessary to fractionate the phosphopeptides. However …

Protein phosphorylation serves as a primary mechanism of signal transduction in the cells of
biological organisms. Technical advancements over the last several years in mass …