Deep learning-based point-scanning super-resolution imaging
Point-scanning imaging systems are among the most widely used tools for high-resolution
cellular and tissue imaging, benefiting from arbitrarily defined pixel sizes. The resolution …
cellular and tissue imaging, benefiting from arbitrarily defined pixel sizes. The resolution …
Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy
Bacterial biofilms represent a basic form of multicellular organization that confers survival
advantages to constituent cells. The sequential stages of cell ordering during biofilm …
advantages to constituent cells. The sequential stages of cell ordering during biofilm …
Concordance of MERFISH spatial transcriptomics with bulk and single-cell RNA sequencing
Spatial transcriptomics extends single-cell RNA sequencing (scRNA-seq) by providing
spatial context for cell type identification and analysis. Imaging-based spatial technologies …
spatial context for cell type identification and analysis. Imaging-based spatial technologies …
Structural and developmental principles of neuropil assembly in C. elegans
Neuropil is a fundamental form of tissue organization within the brain, in which densely
packed neurons synaptically interconnect into precise circuit architecture,. However, the …
packed neurons synaptically interconnect into precise circuit architecture,. However, the …
Accurate and rapid background estimation in single-molecule localization microscopy using the deep neural network BGnet
Background fluorescence, especially when it exhibits undesired spatial features, is a primary
factor for reduced image quality in optical microscopy. Structured background is particularly …
factor for reduced image quality in optical microscopy. Structured background is particularly …
Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association
AR Bhagwat, V Le Sage, E Nturibi, K Kulej… - Nature …, 2020 - nature.com
Assembly of infectious influenza A viruses (IAV) is a complex process involving transport
from the nucleus to the plasma membrane. Rab11A-containing recycling endosomes have …
from the nucleus to the plasma membrane. Rab11A-containing recycling endosomes have …
Accurate phase retrieval of complex 3D point spread functions with deep residual neural networks
Phase retrieval, ie, the reconstruction of phase information from intensity information, is a
central problem in many optical systems. Imaging the emission from a point source such as …
central problem in many optical systems. Imaging the emission from a point source such as …
Zebrafish Posterior Lateral Line primordium migration requires interactions between a superficial sheath of motile cells and the skin
The Zebrafish Posterior Lateral Line primordium migrates in a channel between the skin and
somites. Its migration depends on the coordinated movement of its mesenchymal-like …
somites. Its migration depends on the coordinated movement of its mesenchymal-like …
Transforming the development and dissemination of cutting-edge microscopy and computation
We propose a network of national imaging centers that provide collaborative,
interdisciplinary spaces needed for the development, application, and teaching of advanced …
interdisciplinary spaces needed for the development, application, and teaching of advanced …
Light-sheet fluorescence imaging charts the gastrula origin of vascular endothelial cells in early zebrafish embryos
It remains challenging to construct a complete cell lineage map of the origin of vascular
endothelial cells in any vertebrate embryo. Here, we report the application of in toto light …
endothelial cells in any vertebrate embryo. Here, we report the application of in toto light …