As the COVID-19 pandemic continues to affect human health across the globe rapid, simple,
point-of-care (POC) diagnosis of infectious viruses such as SARS-CoV-2 remains …

Duchenne muscular dystrophy (DMD) is a severe neuromuscular disease arising from loss-
of-function mutations in the dystrophin gene and characterized by progressive muscle …

CRISPR-Cas12a nucleases have expanded the toolbox for targeted genome engineering in
a broad range of organisms. Here, using a high-throughput engineering approach, we …

The Lachnospiraceae is a family of anaerobic bacteria in the class Clostridia with potential
to advance the bio-economy and intestinal therapeutics. Some species of Lachnospiraceae …

Cas12a is an RNA-guided endonuclease that has been widely used for convenient
multiplex gene editing with low off-target effects. To minimize off-targeting in gene editing …

Main conclusion The enhancement of CRISPR-Cas gene editing with robust nuclease
activity promotes genetic modification of desirable agronomic traits, such as resistance to …

CRISPR/Cas12a 系统能够由可编程的RNA 引导, 准确识别特异的单链DNA 或含有PAM
序列的双链DNA, 而后在目标底物进行切割的同时完成对非特异性单链DNA 的迅速切割 …

Abstract Background The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-
editing tool with properties distinct from the broadly used Cas9 system. Features such as …

Nocardia cyriacigeorgica has gradually become a common pathogen in clinical microbial
infections. Identification of Nocardia at the species level is essential to assess the …

The properties of Cas12a nucleases constrict the range of accessible targets and their
applications. In this study, we applied ancestral sequence reconstruction (ASR) to a set of …