Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

H Zhang, L Cao, J Brodsky, I Gablech, F Xu, Z Li… - TrAC Trends in …, 2024 - Elsevier
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …

Precise digital bacteria enumeration and antibiotic susceptibility testing via a portable vibrating capillary-based droplet platform

C Ding, Y Liu, Y Guo, X Guo, Q Kang, X Yan… - Sensors and Actuators B …, 2023 - Elsevier
Antibiotic resistance can lead to multidrug-resistant superbugs and threaten global health,
putting forward a high demand for accurate and fast antibiotic susceptibility testing (AST) …

Deep learning-assisted droplet digital PCR for quantitative detection of human coronavirus

YS Lee, JW Choi, T Kang, BG Chung - BioChip Journal, 2023 - Springer
Abstract Since coronavirus disease 2019 (COVID-19) pandemic rapidly spread worldwide,
there is an urgent demand for accurate and suitable nucleic acid detection technology …

Compact wide-field femtoliter-chamber imaging system for high-speed and accurate digital bioanalysis

T Iida, J Ando, H Shinoda, A Makino, M Yoshimura… - Lab on a Chip, 2023 - pubs.rsc.org
The femtoliter-chamber array is a bioanalytical platform that enables highly sensitive and
quantitative analysis of biological reactions at the single-molecule level. This feature has …

A one-stage deep learning based method for automatic analysis of droplet-based digital PCR images

Y Yao, S Zhao, Y Liang, F Hu, N Peng - Analyst, 2023 - pubs.rsc.org
Droplet-based dPCR offers many advantages over chip-based dPCR, such as lower
processing cost, higher droplet density, higher throughput, while requiring less sample …

[HTML][HTML] Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings

A Mirabile, G Sangiorgio, PG Bonacci, D Bivona… - Diagnostics, 2024 - mdpi.com
Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in
molecular biology and diagnostics, offering exceptional precision and sensitivity in nucleic …

Smartphone integrated handheld (SPEED) digital polymerase chain reaction device

X Liu, X Wang, H Zhang, Z Yan, M Gaňová… - Biosensors and …, 2023 - Elsevier
We demonstrate a smartphone integrated handheld (SPEED) digital polymerase chain
reaction (dPCR) device for point-of-care application. The device has dimensions of≈ 100× …

Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

AS Yadav, FM Galogahi, A Vashi, CH Ooi, KR Sreejith… - Lab on a Chip, 2023 - pubs.rsc.org
Digital droplet reactors have become a valuable tool for the analysis of single cells,
organisms, or molecules by discretising reagents into picolitre or nanolitre volumes …

An approach for integrating droplet generation and detection in digital polymerase chain reaction applications based on a bifunctional microfluidic cross-structure

J Wang, X Lyu, X Zhang, S Wang, W Zeng, T Yang… - Talanta, 2024 - Elsevier
Digital polymerase chain reaction (dPCR) is an approach for absolute nucleic acid
quantification with high sensitivity. Although several successful commercial dPCR devices …

Digital polymerase chain reaction duplexing method in a single fluorescence channel

H Zhang, S Laššáková, Z Yan, X Wang, P Šenkyřík… - Analytica Chimica …, 2023 - Elsevier
The digital polymerase chain reaction (dPCR) technique can quantify specific sequences of
deoxyribonucleic acid using either a droplet-based or chip-based system. dPCR duplexing …