Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …
Precise digital bacteria enumeration and antibiotic susceptibility testing via a portable vibrating capillary-based droplet platform
C Ding, Y Liu, Y Guo, X Guo, Q Kang, X Yan… - Sensors and Actuators B …, 2023 - Elsevier
Antibiotic resistance can lead to multidrug-resistant superbugs and threaten global health,
putting forward a high demand for accurate and fast antibiotic susceptibility testing (AST) …
putting forward a high demand for accurate and fast antibiotic susceptibility testing (AST) …
Deep learning-assisted droplet digital PCR for quantitative detection of human coronavirus
Abstract Since coronavirus disease 2019 (COVID-19) pandemic rapidly spread worldwide,
there is an urgent demand for accurate and suitable nucleic acid detection technology …
there is an urgent demand for accurate and suitable nucleic acid detection technology …
Compact wide-field femtoliter-chamber imaging system for high-speed and accurate digital bioanalysis
The femtoliter-chamber array is a bioanalytical platform that enables highly sensitive and
quantitative analysis of biological reactions at the single-molecule level. This feature has …
quantitative analysis of biological reactions at the single-molecule level. This feature has …
A one-stage deep learning based method for automatic analysis of droplet-based digital PCR images
Y Yao, S Zhao, Y Liang, F Hu, N Peng - Analyst, 2023 - pubs.rsc.org
Droplet-based dPCR offers many advantages over chip-based dPCR, such as lower
processing cost, higher droplet density, higher throughput, while requiring less sample …
processing cost, higher droplet density, higher throughput, while requiring less sample …
[HTML][HTML] Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings
A Mirabile, G Sangiorgio, PG Bonacci, D Bivona… - Diagnostics, 2024 - mdpi.com
Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in
molecular biology and diagnostics, offering exceptional precision and sensitivity in nucleic …
molecular biology and diagnostics, offering exceptional precision and sensitivity in nucleic …
Smartphone integrated handheld (SPEED) digital polymerase chain reaction device
X Liu, X Wang, H Zhang, Z Yan, M Gaňová… - Biosensors and …, 2023 - Elsevier
We demonstrate a smartphone integrated handheld (SPEED) digital polymerase chain
reaction (dPCR) device for point-of-care application. The device has dimensions of≈ 100× …
reaction (dPCR) device for point-of-care application. The device has dimensions of≈ 100× …
Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction
Digital droplet reactors have become a valuable tool for the analysis of single cells,
organisms, or molecules by discretising reagents into picolitre or nanolitre volumes …
organisms, or molecules by discretising reagents into picolitre or nanolitre volumes …
An approach for integrating droplet generation and detection in digital polymerase chain reaction applications based on a bifunctional microfluidic cross-structure
J Wang, X Lyu, X Zhang, S Wang, W Zeng, T Yang… - Talanta, 2024 - Elsevier
Digital polymerase chain reaction (dPCR) is an approach for absolute nucleic acid
quantification with high sensitivity. Although several successful commercial dPCR devices …
quantification with high sensitivity. Although several successful commercial dPCR devices …
Digital polymerase chain reaction duplexing method in a single fluorescence channel
H Zhang, S Laššáková, Z Yan, X Wang, P Šenkyřík… - Analytica Chimica …, 2023 - Elsevier
The digital polymerase chain reaction (dPCR) technique can quantify specific sequences of
deoxyribonucleic acid using either a droplet-based or chip-based system. dPCR duplexing …
deoxyribonucleic acid using either a droplet-based or chip-based system. dPCR duplexing …