A fundamental challenge for the survival of all organisms is maintaining the integrity of the
genome in all cells. Cells must therefore segregate their replicated genome equally during …

Centromeric chromatin is distinguished primarily by nucleosomes containing the histone
variant cenH3, which organizes the kinetochore that links the chromosome to the spindle …

The centromere, responsible for chromosome segregation during mitosis, is epigenetically
defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct …

Abstract The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved
kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for …

Kinetochores are megadalton-sized protein complexes that mediate chromosome–
microtubule interactions in eukaryotes. How kinetochore assembly is triggered specifically …

The budding yeast centromere contains Cse4, a specialized histone H3 variant.
Fluorescence pulse-chase analysis of an internally tagged Cse4 reveals that it is replaced …

In budding yeast, a single cenH3 (Cse4) nucleosome occupies the∼ 120-bp functional
centromere, however conflicting structural models for the particle have been proposed. To …

Centromeres are specialized domains of heterochromatin that provide the foundation for the
kinetochore. Centromeric heterochromatin is characterized by specific histone modifications …

The centromere is the genetic locus that specifies the site of kinetochore assembly, where
the chromosome will attach to the kinetochore microtubule. The pericentromere is the …

Proper chromosome segregation is crucial for cell division. In eukaryotes, this is achieved by
the kinetochore, an evolutionarily conserved multiprotein complex that physically links the …