Challenges in the use of sortase and other peptide ligases for site-specific protein modification
HE Morgan, WB Turnbull, ME Webb - Chemical Society Reviews, 2022 - pubs.rsc.org
Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …
many challenges associated with the development of protein modification techniques, in …
Recent progress in enzymatic protein labelling techniques and their applications
Protein-based conjugates are valuable constructs for a variety of applications. Conjugation
of proteins to fluorophores is commonly used to study their cellular localization and the …
of proteins to fluorophores is commonly used to study their cellular localization and the …
Sortase A: a model for transpeptidation and its biological applications
N Pishesha, JR Ingram… - Annual review of cell and …, 2018 - annualreviews.org
Molecular biologists and chemists alike have long sought to modify proteins with
substituents that cannot be installed by standard or even advanced genetic approaches. We …
substituents that cannot be installed by standard or even advanced genetic approaches. We …
Machine-learning-guided library design cycle for directed evolution of enzymes: the effects of training data composition on sequence space exploration
Y Saito, M Oikawa, T Sato, H Nakazawa, T Ito… - Acs …, 2021 - ACS Publications
Machine learning (ML) is becoming an attractive tool in mutagenesis-based protein
engineering because of its ability to design a variant library containing proteins with a …
engineering because of its ability to design a variant library containing proteins with a …
Recent advances in sortase-catalyzed ligation methodology
JM Antos, MC Truttmann, HL Ploegh - Current opinion in structural biology, 2016 - Elsevier
Highlights•Sortase-catalyzed ligation is a powerful strategy for protein modification.•
Engineered sortases improve reaction rates and eliminate Ca 2+ dependency.•Deactivation …
Engineered sortases improve reaction rates and eliminate Ca 2+ dependency.•Deactivation …
Emerging enzymes for ATP regeneration in biocatalytic processes
JN Andexer, M Richter - ChemBioChem, 2015 - Wiley Online Library
Abstract Adenosine‐5′‐triphosphate‐dependent enzyme catalysed reactions are
widespread in nature. Consequently, the enzymes involved have an intrinsic potential for …
widespread in nature. Consequently, the enzymes involved have an intrinsic potential for …
Research progress in enzymatically cross-linked hydrogels as injectable systems for bioprinting and tissue engineering
R Naranjo-Alcazar, S Bendix, T Groth, G Gallego Ferrer - Gels, 2023 - mdpi.com
Hydrogels have been developed for different biomedical applications such as in vitro culture
platforms, drug delivery, bioprinting and tissue engineering. Enzymatic cross-linking has …
platforms, drug delivery, bioprinting and tissue engineering. Enzymatic cross-linking has …
Molecular features of the sortase enzyme family
WJ Bradshaw, AH Davies, CJ Chambers… - The FEBS …, 2015 - Wiley Online Library
Bacteria possess complex and varying cell walls with many surface exposed proteins.
Sortases are responsible for the covalent attachment of specific proteins to the …
Sortases are responsible for the covalent attachment of specific proteins to the …
Empowering Site‐Specific Bioconjugations In Vitro and In Vivo: Advances in Sortase Engineering and Sortase‐Mediated Ligation
Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …
Anti-SARS-CoV-1 and− 2 nanobody engineering towards avidity-inspired therapeutics
In the past two decades, the emergence of coronavirus diseases has been dire distress on
both continental and global fronts and has resulted in the search for potent treatment …
both continental and global fronts and has resulted in the search for potent treatment …