Challenges in the use of sortase and other peptide ligases for site-specific protein modification

HE Morgan, WB Turnbull, ME Webb - Chemical Society Reviews, 2022 - pubs.rsc.org
Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Recent progress in enzymatic protein labelling techniques and their applications

Y Zhang, KY Park, KF Suazo… - Chemical Society Reviews, 2018 - pubs.rsc.org
Protein-based conjugates are valuable constructs for a variety of applications. Conjugation
of proteins to fluorophores is commonly used to study their cellular localization and the …

Sortase A: a model for transpeptidation and its biological applications

N Pishesha, JR Ingram… - Annual review of cell and …, 2018 - annualreviews.org
Molecular biologists and chemists alike have long sought to modify proteins with
substituents that cannot be installed by standard or even advanced genetic approaches. We …

Machine-learning-guided library design cycle for directed evolution of enzymes: the effects of training data composition on sequence space exploration

Y Saito, M Oikawa, T Sato, H Nakazawa, T Ito… - Acs …, 2021 - ACS Publications
Machine learning (ML) is becoming an attractive tool in mutagenesis-based protein
engineering because of its ability to design a variant library containing proteins with a …

Recent advances in sortase-catalyzed ligation methodology

JM Antos, MC Truttmann, HL Ploegh - Current opinion in structural biology, 2016 - Elsevier
Highlights•Sortase-catalyzed ligation is a powerful strategy for protein modification.•
Engineered sortases improve reaction rates and eliminate Ca 2+ dependency.•Deactivation …

Emerging enzymes for ATP regeneration in biocatalytic processes

JN Andexer, M Richter - ChemBioChem, 2015 - Wiley Online Library
Abstract Adenosine‐5′‐triphosphate‐dependent enzyme catalysed reactions are
widespread in nature. Consequently, the enzymes involved have an intrinsic potential for …

Research progress in enzymatically cross-linked hydrogels as injectable systems for bioprinting and tissue engineering

R Naranjo-Alcazar, S Bendix, T Groth, G Gallego Ferrer - Gels, 2023 - mdpi.com
Hydrogels have been developed for different biomedical applications such as in vitro culture
platforms, drug delivery, bioprinting and tissue engineering. Enzymatic cross-linking has …

Molecular features of the sortase enzyme family

WJ Bradshaw, AH Davies, CJ Chambers… - The FEBS …, 2015 - Wiley Online Library
Bacteria possess complex and varying cell walls with many surface exposed proteins.
Sortases are responsible for the covalent attachment of specific proteins to the …

Empowering Site‐Specific Bioconjugations In Vitro and In Vivo: Advances in Sortase Engineering and Sortase‐Mediated Ligation

Z Zou, Y Ji, U Schwaneberg - … Chemie International Edition, 2024 - Wiley Online Library
Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

Anti-SARS-CoV-1 and− 2 nanobody engineering towards avidity-inspired therapeutics

EM Obeng, CKO Dzuvor, MK Danquah - Nano Today, 2022 - Elsevier
In the past two decades, the emergence of coronavirus diseases has been dire distress on
both continental and global fronts and has resulted in the search for potent treatment …