Functional metagenomics for enzyme discovery: challenges to efficient screening

T Uchiyama, K Miyazaki - Current opinion in biotechnology, 2009 - Elsevier
Metagenomics has emerged as an alternative approach to conventional microbial screening
that allows exhaustive screening of microbial genomes in their natural environments …

The great screen anomaly—a new frontier in product discovery through functional metagenomics

DM Ekkers, MS Cretoiu, AM Kielak… - Applied microbiology and …, 2012 - Springer
Functional metagenomics, the study of the collective genome of a microbial community by
expressing it in a foreign host, is an emerging field in biotechnology. Over the past years, the …

Changes in the proteome of Salmonella enterica serovar Thompson as stress adaptation to sublethal concentrations of thymol

R Di Pasqua, G Mamone, P Ferranti, D Ercolini… - …, 2010 - Wiley Online Library
Thymol is a natural biocide and component of some essential oils from herbs. Its inhibitory
effect on the growth of different microorganisms is well documented. The precise targets of …

Ribosomal protein S1 unwinds double-stranded RNA in multiple steps

X Qu, L Lancaster, HF Noller… - Proceedings of the …, 2012 - National Acad Sciences
The sequence and secondary structure of the 5′-end of mRNAs regulate translation by
controlling ribosome initiation on the mRNA. Ribosomal protein S1 is crucial for ribosome …

Probing the relationship between Gram-negative and Gram-positive S1 proteins by sequence analysis

P Salah, M Bisaglia, P Aliprandi, M Uzan… - Nucleic acids …, 2009 - academic.oup.com
Escherichia coli ribosomal protein S1 is required for the translation initiation of messenger
RNAs, in particular when their Shine–Dalgarno sequence is degenerated. Closely related …

Multiple activities of RNA-binding proteins S1 and Hfq

E Hajnsdorf, IV Boni - Biochimie, 2012 - Elsevier
In all organisms, RNA-binding proteins participate in modulating all the steps in the life cycle
of RNA, including transcription, folding, translation and turnover. In bacteria, RNA-binding …

Bioprospection of marine microorganisms: biotechnological applications and methods

HM Dionisi, M Lozada, NL Olivera - Revista argentina de …, 2012 - SciELO Argentina
Bioprospection of marine microorganisms: biotechnological applications and methods SciELO
- Scientific Electronic Library Online vol.44 número1 Predación selectiva de bacterias …

Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries

SM Gaida, NR Sandoval, SA Nicolaou, Y Chen… - Nature …, 2015 - nature.com
A key limitation in using heterologous genomic or metagenomic libraries in functional
genomics and genome engineering is the low expression of heterologous genes in …

Mining Novel Constitutive Promoter Elements in Soil Metagenomic Libraries in Escherichia coli

CA Westmann, LF Alves, R Silva-Rocha… - Frontiers in …, 2018 - frontiersin.org
Although functional metagenomics has been widely employed for the discovery of genes
relevant to biotechnology and biomedicine, its potential for assessing the diversity of …

S1 ribosomal protein functions in translation initiation and ribonuclease RegB activation are mediated by similar RNA-protein interactions: an NMR and SAXS analysis

P Aliprandi, C Sizun, J Perez, F Mareuil… - Journal of Biological …, 2008 - ASBMB
The ribosomal protein S1, in Escherichia coli, is necessary for the recognition by the
ribosome of the translation initiation codon of most messenger RNAs. It also participates in …