Cryo-EM of the Nucleosome Core Particle Bound to Ran-RCC1 Reveals a Dynamic Complex

SK Huang, JL Rubinstein, LE Kay - Biochemistry, 2024 - ACS Publications
Ras-related nuclear protein (Ran) is a member of the Ras superfamily of small guanosine
triphosphatases (GTPases) and a regulator of multiple cellular processes. In healthy cells …

Molecular basis of RanGTP-activated release of Histones H2A-H2B from Importin-9

JM Shaffer, J Jiou, K Tripathi, OS Olaluwoye, HYJ Fung… - Structure, 2023 - cell.com
Imp9 is the primary importin for shuttling H2A-H2B from the cytoplasm to the nucleus. It
employs an unusual mechanism where the binding of RanGTP is insufficient to release H2A …

A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 linked to neurodevelopmental disorders

A Gonzalez, HJ Kim, BD Freibaum, HYJ Fung… - Structure, 2023 - cell.com
The HNRNPH2 proline-tyrosine nuclear localization signal (PY-NLS) is mutated in
HNRNPH2-related X-linked neurodevelopmental disorder, causing the normally nuclear …

Nap1 and Kap114 co-chaperone H2A-H2B and facilitate targeted histone release in the nucleus

HYJ Fung, J Jiou, AB Niesman, NE Bernardes… - Journal of Cell …, 2025 - rupress.org
Core histones, synthesized and processed in the cytoplasm, must be chaperoned as they
are transported into the nucleus for nucleosome assembly. The importin Kap114 transports …