Analysing microbial community composition through amplicon sequencing: from sampling to hypothesis testing

LW Hugerth, AF Andersson - Frontiers in microbiology, 2017 - frontiersin.org
Microbial ecology as a scientific field is fundamentally driven by technological advance. The
past decade's revolution in DNA sequencing cost and throughput has made it possible for …

Microbial indicators of fecal pollution: recent progress and challenges in assessing water quality

DA Holcomb, JR Stewart - Current environmental health reports, 2020 - Springer
Abstract Purpose of Review Fecal contamination of water is a major public health concern.
This review summarizes recent developments and advancements in water quality indicators …

Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies

V Sevim, J Lee, R Egan, A Clum, H Hundley, J Lee… - Scientific data, 2019 - nature.com
Metagenomic sequence data from defined mock communities is crucial for the assessment
of sequencing platform performance and downstream analyses, including assembly, binning …

[HTML][HTML] Metagenomic water quality monitoring with a portable laboratory

K Acharya, A Blackburn, J Mohammed, AT Haile… - Water research, 2020 - Elsevier
We describe the technical feasibility of metagenomic water quality analysis using only
portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges …

Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?

LJ Kerkhof - FEMS microbiology ecology, 2021 - academic.oup.com
This minireview will discuss the improvements in Oxford Nanopore (Oxford; sequencing
technology that make the MinION a viable platform for microbial ecology studies. Specific …

Rapid detection methods for bacterial pathogens in ambient waters at the point of sample collection: a brief review

J Li, Y Zhu, X Wu, MR Hoffmann - Clinical Infectious Diseases, 2020 - academic.oup.com
The world is currently facing a serious health burden of waterborne diseases, including
diarrhea, gastrointestinal diseases, and systemic illnesses. The control of these infectious …

[HTML][HTML] Environmental DNA clarifies impacts of combined sewer overflows on the bacteriology of an urban river and resulting risks to public health

R Zan, A Blackburn, J Plaimart, K Acharya… - Science of the Total …, 2023 - Elsevier
There is no reference of microbiological water quality in the European Union's Water
Framework Directive, adapted into English law, and consequently microbial water quality is …

Decay of four enteric pathogens and implications to wastewater-based epidemiology: Effects of temperature and wastewater dilutions

Y Guo, M Sivakumar, G Jiang - Science of The Total Environment, 2022 - Elsevier
Measurement of pathogens in raw wastewater from a population within certain sewer
catchments can provide quantitative information on public health status within the sampled …

A comparative assessment of conventional and molecular methods, including MinION nanopore sequencing, for surveying water quality

K Acharya, S Khanal, K Pantha, N Amatya… - Scientific reports, 2019 - nature.com
Nucleic acid based techniques, such as quantitative PCR (qPCR) and next generation
sequencing (NGS), provide new insights into microbial water quality, but considerable …

MinION Nanopore sequencing accelerates progress towards ubiquitous genetics in water research

D Werner, K Acharya, A Blackburn, R Zan, J Plaimart… - Water, 2022 - mdpi.com
In 2014, Oxford Nanopore Technologies (ONT) introduced an affordable and portable
sequencer called MinION. We reviewed emerging applications in water research and …