A decade of advances in transposon-insertion sequencing

AK Cain, L Barquist, AL Goodman, IT Paulsen… - Nature Reviews …, 2020 - nature.com
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

[HTML][HTML] The vulnerable versatility of Salmonella antibiotic persisters during infection

PWS Hill, AL Moldoveanu, M Sargen, S Ronneau… - Cell Host & Microbe, 2021 - cell.com
Tolerance and persistence are superficially similar phenomena by which bacteria survive
bactericidal antibiotics. It is assumed that the same physiology underlies survival of …

Shedding light on bacteria–host interactions with the aid of TnSeq approaches

M Torres, S Paszti, L Eberl - Mbio, 2024 - Am Soc Microbiol
Bacteria are highly adaptable and grow in diverse niches, where they often interact with
eukaryotic organisms. These interactions with different hosts span the entire spectrum from …

Structure, biosynthesis and regulation of the T1 antigen, a phase-variable surface polysaccharide conserved in many Salmonella serovars

SD Kelly, MJ Allas, LD Goodridge, TL Lowary… - Nature …, 2024 - nature.com
The bacterial genus Salmonella includes diverse isolates with multiple variations in the
structure of the main polysaccharide component (O antigen) of membrane …

Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections

V García, RB Grønnemose… - Microbial …, 2021 - microbiologyresearch.org
Uropathogenic Escherichia coli (UPEC) UTI89 is a well-characterized strain, which has
mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little …

High-throughput fitness experiments reveal specific vulnerabilities of human-adapted Salmonella during stress and infection

BX Wang, D Leshchiner, L Luo, M Tuncel, K Hokamp… - Nature Genetics, 2024 - nature.com
Salmonella enterica is comprised of genetically distinct 'serovars' that together provide an
intriguing model for exploring the genetic basis of pathogen evolution. Although the …

Genome-wide fitness analysis of Salmonella enterica reveals aroA mutants are attenuated due to iron restriction in vitro

JL Rooke, ECA Goodall, K Pullela, R Da Costa… - Mbio, 2024 - journals.asm.org
Salmonella enterica is a globally disseminated pathogen that is the cause of over 100
million infections per year. The resulting diseases are dependent upon host susceptibility …

Genetic variation in the MacAB-TolC efflux pump influences pathogenesis of invasive Salmonella isolates from Africa

JD Honeycutt, N Wenner, Y Li, SM Brewer… - PLoS …, 2020 - journals.plos.org
The various sub-species of Salmonella enterica cause a range of disease in human hosts.
The human-adapted Salmonella enterica serovar Typhi enters the gastrointestinal tract and …

A global data-driven census of Salmonella small proteins and their potential functions in bacterial virulence

E Venturini, SL Svensson, S Maaß, R Gelhausen… - …, 2020 - academic.oup.com
Small proteins are an emerging class of gene products with diverse roles in bacterial
physiology. However, a full understanding of their importance has been hampered by …

Gene essentiality profiling reveals a novel determinant of stresses preventing protein aggregation in Salmonella

Z Wang, S Zhu, C Li, L Lyu, J Yu, D Wang… - Emerging Microbes & …, 2022 - Taylor & Francis
Adaptation to various stresses during infection is important for Salmonella Typhimurium
virulence, while the fitness determinants under infection-relevant stress conditions remain …