Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

Various non-nucleic acid targets (ions, small molecules, polysaccharides, peptides/proteins/
enzymes, cells, transcription factors) are important biomarkers. They play important roles in …

Exosomal proteins are considered to be promising indicators of cancer. Herein, a novel
DNAzyme walkers-triggered CRISPR-Cas12a/Cas13a strategy was proposed for the …

This paper reviews methods for detecting proteins based on molecular digitization, ie, the
isolation and detection of single protein molecules or singulated ensembles of protein …

Accurate diagnosis of human diseases is crucial for effective prevention, clinical treatment,
and prognosis. The World Health Organization (WHO) has provided guidelines for molecular …

Biosensing based on CRISPR-Cas systems is a young but rapidly evolving technology. The
unprecedented properties of the CRISPR-Cas system provide an innovative tool for …

Growing studies focusing on nuclear acid detection via the emerging CRISPR technique
demonstrate its promising application. However, limited works solve the identification of non …

Recent advances in CRISPR-based biotechnologies have greatly expanded our capabilities
to repurpose CRISPR for the development of molecular diagnostic systems. The key …

The level of Interleukin-4 (IL-4, a classical anti-inflammatory cytokine) in cerebrospinal fluid
is in response to brain injury, infection and neurodegenerative diseases. For early …

Rapid, accurate, and noninvasive detection of biomarkers in saliva, urine, or nasal fluid is
essential for the identification, early diagnosis, and monitoring of cancer, organ failure …