Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing

Y Wang, Q Fu, SY Park, YS Lee, SY Park, DY Lee… - Biotechnology …, 2024 - Elsevier
Recombinant adeno-associated virus (rAAV) is one of the prominent gene delivery vehicles
that has opened promising opportunities for novel gene therapeutic approaches. However …

[HTML][HTML] Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms

D Catalán-Tatjer, K Tzimou, LK Nielsen… - Biotechnology …, 2024 - Elsevier
Recombinant adeno-associated viruses (rAAVs) stand at the forefront of gene therapy
applications, holding immense significance for their safe and efficient gene delivery …

The downstream bioprocess toolbox for therapeutic viral vectors

R Kilgore, A Minzoni, S Shastry, W Smith… - … of Chromatography A, 2023 - Elsevier
Viral vectors are poised to acquire a prominent position in modern medicine and
biotechnology owing to their role as delivery agents for gene therapies, oncolytic agents …

High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions

L Coplan, Z Zhang, N Ragone, J Reeves… - Biotechnology …, 2024 - Wiley Online Library
Recombinant adeno‐associated viral vectors (rAAVs) are one of the most used vehicles for
gene therapy, with five rAAV therapeutics commercially approved by the FDA. To improve …

Enhanced ER protein processing gene expression increases rAAV yield and full capsid ratio in HEK293 cells

Q Fu, Y Wang, J Qin, D Xie, D McNally… - Applied Microbiology and …, 2024 - Springer
The recombinant adeno-associated virus (rAAV) vector is among the most promising viral
vectors in gene therapy. However, the limited manufacturing capacity in human embryonic …

HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing

SGR Gurazada, HM Kennedy, RD Braatz… - Biotechnology …, 2024 - Elsevier
Gene therapy is poised to transition from niche to mainstream medicine, with recombinant
adeno-associated virus (rAAV) as the vector of choice. However, robust, scalable …

Miniaturization of hiPSC-derived 3D neural cultures in stirred-tank bioreactors for parallelized preclinical assessment of rAAV

CM Gomes, MJ Sebastião, G Silva, F Moura… - … in Bioengineering and …, 2024 - frontiersin.org
Introduction: Engineered 3D models employing human induced pluripotent stem cell
(hiPSC) derivatives have the potential to recapitulate the cell diversity and structure found in …

Challenges and Solutions in the Recombinant Expression of Membrane Proteins

C Liu, H He, J Tian, Y Ma - Protein and Peptide Letters, 2023 - benthamdirect.com
Membrane proteins are important components of the proteome and play key roles in many
biological processes, such as signal transduction, material transport, cell recognition, etc …

Accelerated Development of AAV Purification Process Using a High-Throughput and Automated Crossflow System

S Mendes, TQ Faria, A Nascimento, M Noverraz… - Separations, 2024 - mdpi.com
Adeno-associated viruses (AAV) are currently predominant viral transfer tools for gene
therapy, and efforts are being made to design faster and more efficient methods and …

[引用][C] Advanced cell technologies: Making protein, cell, and gene therapies a reality

I Geada, A Roldão, MJ Betenbaugh… - Biotechnology and …, 2023 - Wiley Online Library
Researchers and engineers came together in Lisbon at the 27th Meeting of the European
Society for Animal Cell Technology (ESACT 2022), to discuss the latest advances in …