Three-dimensional organotypic culture: experimental models of mammalian biology and disease
Mammalian organs are challenging to study as they are fairly inaccessible to experimental
manipulation and optical observation. Recent advances in three-dimensional (3D) culture …
manipulation and optical observation. Recent advances in three-dimensional (3D) culture …
Matrigel: from discovery and ECM mimicry to assays and models for cancer research
G Benton, I Arnaoutova, J George, HK Kleinman… - Advanced drug delivery …, 2014 - Elsevier
The basement membrane is an important extracellular matrix that is found in all epithelial
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …
Matrigel: basement membrane matrix with biological activity
HK Kleinman, GR Martin - Seminars in cancer biology, 2005 - Elsevier
The basement membrane extracellular matrix contacts epithelial, endothelial, fat and smooth
muscle cells. Because this extracellular matrix is so thin, it had been hard to study its …
muscle cells. Because this extracellular matrix is so thin, it had been hard to study its …
In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract
I Arnaoutova, HK Kleinman - Nature protocols, 2010 - nature.com
A protocol is presented here for a rapid, quantitative and reliable in vitro angiogenesis assay
that can be adapted for high throughput use. Endothelial cells are plated on a gelled …
that can be adapted for high throughput use. Endothelial cells are plated on a gelled …
Cadherin expression in carcinomas: role in the formation of cell junctions and the prevention of invasiveness
W Birchmeier, J Behrens - Biochimica et Biophysica Acta (BBA)-Reviews …, 1994 - Elsevier
Cell adhesion in tumors............................................. 1.1. Invasive carcinoma cells exhibit
reduced intercellular adhesion.................... 1.2. The role of the cell adhesion molecule E …
reduced intercellular adhesion.................... 1.2. The role of the cell adhesion molecule E …
Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix …
S Vukicevic, HK Kleinman, FP Luyten… - Experimental cell …, 1992 - Elsevier
We have recently demonstrated the formation of interconnecting canalicular cell processes
in bone cells upon contact with basement membrane components. Here we have …
in bone cells upon contact with basement membrane components. Here we have …
Modeling pancreatic cancer with organoids
Pancreatic ductal adenocarcinoma (PDA) is a highly lethal malignancy for which new
treatment and diagnostic approaches are urgently needed. For such breakthroughs to be …
treatment and diagnostic approaches are urgently needed. For such breakthroughs to be …
The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art
I Arnaoutova, J George, HK Kleinman, G Benton - Angiogenesis, 2009 - Springer
It has been more than 20 years since it was first demonstrated that endothelial cells will
rapidly form capillary-like structures in vitro when plated on top of a reconstituted basement …
rapidly form capillary-like structures in vitro when plated on top of a reconstituted basement …
Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix
N Boudreau, CJ Sympson, Z Werb, MJ Bissell - Science, 1995 - science.org
Apoptosis (programmed cell death) plays a major role in development and tissue
regeneration. Basement membrane extracellular matrix (ECM), but not fibronectin or …
regeneration. Basement membrane extracellular matrix (ECM), but not fibronectin or …
Hydrogels and dentin–pulp complex regeneration: from the benchtop to clinical translation
Dentin–pulp complex is a term which refers to the dental pulp (DP) surrounded by dentin
along its peripheries. Dentin and dental pulp are highly specialized tissues, which can be …
along its peripheries. Dentin and dental pulp are highly specialized tissues, which can be …