The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …

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The development of imaging techniques beyond the diffraction limit has paved the way for
detailed studies of nanostructures and molecular mechanisms in biological systems …

Confocal microscopy remains a major workhorse in biomedical optical microscopy owing to
its reliability and flexibility in imaging various samples, but suffers from substantial point …

Most fluorescence microscopes are inefficient, collecting only a small fraction of the emitted
light at any instant. Besides wasting valuable signal, this inefficiency also reduces spatial …

Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, and
gentle imaging of live specimens over extended periods. Here we describe a technique that …

Long-term observation of subcellular dynamics in living organisms is limited by background
fluorescence originating from tissue scattering or dense labeling. Existing confocal …

The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark
contrast to the comprehensive anatomical and gene expression datasets already available …

In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet
of light is combined with fast full-frame acquisition. This illumination scheme provides …

Single-molecule localization microscopy (SMLM) can provide nanoscale resolution in thin
samples but has rarely been applied to tissues because of high background from out-of …