Background Accurate and comprehensive annotation of transcript sequences is essential for
transcript quantification and differential gene and transcript expression analysis. Single …

Alternative splicing of messenger RNAs is associated with the evolution of developmentally
complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA …

Reproductive phasiRNAs (phased, small interfering RNAs) are broadly present in
angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt …

Accurate characterisation of splice junctions (SJs) as well as transcription start and end sites
in reference transcriptomes allows precise quantification of transcripts from RNA‐seq data …

Abstract pre-mRNA splicing is a critical feature of eukaryotic gene expression. Both cis-and
trans-splicing rely on accurately recognising splice site sequences by spliceosomal U …

Inadequate reference databases in RNA‐seq analysis can hinder data utilization and
interpretation. In this study, we have successfully constructed a high‐quality reference …

It is increasingly apparent that although different genotypes within a species share “core”
genes, they also contain variable numbers of “specific” genes and different structures of …

To identify novel solutions to improve rice yield under rising temperatures, molecular
components of thermotolerance must be better understood. Alternative splicing (AS) is a …

The increasing availability of massive omics data requires improving the quality of reference
databases and their annotations. The combination of full-length isoform sequencing (Iso …

HPODE hydroperoxide-octadecadienoic acid HPOT hydroperoxy-octadecatrienoic acid
HSD hydroxysteroid dehydrogenase, steroleosin iBAQ intensity-based absolute …