Single-cell and spatial multi-omics in the plant sciences: Technical advances, applications, and perspectives

X Yu, Z Liu, X Sun - Plant communications, 2023 - cell.com
Plants contain a large number of cell types and exhibit complex regulatory mechanisms.
Studies at the single-cell level have gradually become more common in plant science …

Introducing single cell stereo-sequencing technology to transform the plant transcriptome landscape

G Bawa, Z Liu, X Yu, LSP Tran, X Sun - Trends in Plant Science, 2024 - cell.com
Single cell RNA-sequencing (scRNA-seq) advancements have helped detect transcriptional
heterogeneities in biological samples. However, scRNA-seq cannot currently provide high …

An in situ sequencing approach maps PLASTOCHRON1 at the boundary between indeterminate and determinate cells

R Laureyns, J Joossens, D Herwegh… - Plant …, 2022 - academic.oup.com
The plant shoot apex houses the shoot apical meristem, a highly organized and active stem-
cell tissue where molecular signaling in discrete cells determines when and where leaves …

Spatial transcriptomics of a lycophyte root sheds light on root evolution

X Yang, W Poelmans, C Grones, A Lakehal… - Current Biology, 2023 - cell.com
Plant roots originated independently in lycophytes and euphyllophytes, whereas early
vascular plants were rootless. The organization of the root apical meristem in …

The transcriptome landscape of developing barley seeds

M Kovacik, A Nowicka, J Zwyrtková, B Strejčková… - The Plant …, 2024 - academic.oup.com
Cereal grains are an important source of food and feed. To provide comprehensive
spatiotemporal information about biological processes in developing seeds of cultivated …

Opportunities and Challenges in Advancing Plant Research with Single-cell Omics

MS Rhaman, M Ali, W Ye, B Li - Genomics, Proteomics & …, 2024 - academic.oup.com
Plants possess diverse cell types and intricate regulatory mechanisms to adapt to the ever-
changing environment of nature. Various strategies have been employed to study cell types …

Leaf starch degradation by β-amylase ZmBAM8 influences drought tolerance in maize

L Niu, X Wu, H Liu, X Hu, W Wang - Carbohydrate Polymers, 2024 - Elsevier
As a typical C 4 plant and important crop worldwide, maize is susceptible to drought. In
maize, transitory starch (TS) turnover occurs in the vascular bundle sheath of leaves …

The transcriptome landscape of developing barley seeds reveals H3K27me3 dynamics in endosperm tissues

M Kovacik, A Nowicka, J Zwyrtková, B Strejčková… - bioRxiv, 2023 - biorxiv.org
Cereal grains are an important food and feed. To provide comprehensive spatiotemporal
information about biological processes in developing seeds of cultivated barley, we …

Fluorescence hybridization chain reaction enables localization of multiple molecular classes combined with plant cell ultrastructure

Y Yu, D Huss, M Li, JS Wickramanayake, S Bèlanger… - bioRxiv, 2024 - biorxiv.org
Background Recent developments in hybridization chain reaction (HCR) have enabled
robust simultaneous localization of multiple mRNA transcripts using fluorescence in situ …