Combined Second Harmonic Generation and Fluorescence Analyses of the Structures and Dynamics of Molecules on Lipids Using Dual-Probes: A Review

Y Hou, J Li, B Li, Q Yuan, W Gan - Molecules, 2022 - mdpi.com
Revealing the structures and dynamic behaviors of molecules on lipids is crucial for
understanding the mechanism behind the biophysical processes, such as the preparation …

On the models for understanding the second harmonic generation scattering from lipid membranes and the corresponding molecular kinetics

S Chen, Y Hou, B Xu, S Chen, Q Yuan… - Colloids and Surfaces A …, 2024 - Elsevier
The kinetics of molecular transportation through lipid membranes are key information for
understanding many biophysical processes in real cells or protocells. Second harmonic …

Unveiling the molecular dynamics in a living cell to the subcellular organelle level using second-harmonic generation spectroscopy and microscopy

B Li, J Li, W Gan, Y Tan, Q Yuan - Analytical Chemistry, 2021 - ACS Publications
Second-harmonic generation (SHG) microscopy has been proved to be a powerful method
for investigating the structures of biomaterials. SHG spectra were also generally used to …

AIEgens for dual second harmonic generation and fluorescence “turn-on” imaging of membrane and photodynamic therapy in cancer cells

Y Peng, Y Yan, P Li, B Li, H Jiang, B Guo… - Materials Chemistry …, 2023 - pubs.rsc.org
The integration of second harmonic generation (SHG) microscopic imaging and aggregation-
induced emission fluorescence imaging would greatly boost the imaging outcomes because …

Revealing the mechanisms of vesicle formation with multiple spectral methods

J Li, SL Chen, Y Hou, Q Yuan, W Gan - Physical Chemistry Chemical …, 2022 - pubs.rsc.org
The investigation of the self-assembly of amphiphilic molecules and the formation of
micelles/vesicles has attracted significant attention. However, in situ and real-time methods …

The effect of side group on the dynamic behavior of anthracyclines on DOPG lipid membranes revealed by second harmonic generation and fluorescence

Y Hou, J Li, XX Liu, Y Ruan, S Chen, Q Yuan, W Gan - Chemical Physics, 2021 - Elsevier
In this work we use two-photon fluorescence (TPF) and surface-sensitive second harmonic
generation (SHG) techniques to investigate the interaction of the lipid membrane with …

Probing the structural evolution on the surface of cardiolipin vesicles with an amphiphilic second harmonic generation and fluorescence probe

S Chen, Z Liu, B Li, Y Hou, Y Peng, J Li… - The Journal of …, 2024 - pubs.aip.org
Investigating the influence of the ambient chemical environment on molecular behaviors in
liposomes is crucial for understanding and manipulating cellular vitality as well as the …

Observing the structural variations on binary complex vesicle surfaces and the influence on molecular transportation

Y Ruan, P Guha, SL Chen, Q Yuan, W Gan - Chemical Physics, 2021 - Elsevier
A clear understanding on the structure and functionality of lipid molecules on natural or
artificial cell membranes are important yet hard to be achieved. In this work we compared …

Measuring the activation energy of the structural evolution in vesicle formation with combined spectroscopic methods and revealing the different ionic effects from Na+ …

J Li, B Li, Y Hou, J Zeb, Q Yuan, W Gan - Colloids and Surfaces A …, 2023 - Elsevier
It is not easy to in-situ probe the structural evolution in assembly systems in solutions.
Determining the activation energy of the molecular processes in vesicle formation, which is …

Differences in Lipid Order and Dynamics in Plasma Membranes Assessed by Nonlinear Optical Microscopy

E Aiyama, N Kato - The Journal of Physical Chemistry B, 2024 - ACS Publications
When amphiphilic polar dyes were added to the cells, they intercalated predominantly in the
outer leaf of the plasma membrane, making them active for second harmonic generation …