BACKGROUND Nature has evolved elegant mechanisms to regulate the physical distance
between molecules, or proximity, for a wide variety of purposes. Whether it is activation of …

Auxin is arguably the most important signaling molecule in plants, and the last few decades
have seen remarkable breakthroughs in understanding its production, transport, and …

Protein knockdown using the auxin-inducible degron (AID) technology is useful to study
protein function in living cells because it induces rapid depletion, which makes it possible to …

The CCCTC-binding factor (CTCF) works together with the cohesin complex to drive the
formation of chromatin loops and topologically associating domains, but its role in gene …

INTRODUCTION During mitosis, cells compact their chromosomes into dense rod-shaped
structures to ensure their reliable transmission to daughter cells. Our work explores how …

Methods for the targeted disruption of protein function have revolutionized science and
greatly expedited the systematic characterization of genes. Two main approaches are …

Studying the role of essential proteins is dependent upon a method for rapid inactivation, in
order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) …

INTRODUCTION Detection of pathogens by the innate immune system is an essential first
step in successful host defense against infection. Pathogens are typically detected by …

Experimental manipulation of protein abundance in living cells or organisms is an essential
strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques …

Translation is the fundamental biological process converting mRNA information into
proteins. Single-molecule imaging in live cells has illuminated the dynamics of RNA …