Affinity monolith chromatography: A review of general principles and recent developments

S Poddar, S Sharmeen, DS Hage - Electrophoresis, 2021 - Wiley Online Library
Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a
monolithic support with a biological ligand or related binding agent to isolate, enrich, or …

Current trends in affinity-based monoliths in microextraction approaches: A review

M Vergara-Barberan, EJ Carrasco-Correa… - Analytica Chimica …, 2019 - Elsevier
This article reviews the research contributions along the past five years concerning to
monolithic materials for the development of affinity-based sorbents in the field of …

Characterization of methacrylate chromatographic monoliths bearing affinity ligands

U Černigoj, U Vidic, B Nemec, J Gašperšič… - … of Chromatography A, 2016 - Elsevier
We investigated effect of immobilization procedure and monolith structure on
chromatographic performance of methacrylate monoliths bearing affinity ligands. Monoliths …

High‐throughput immunoaffinity enrichment and N‐glycan analysis of human plasma haptoglobin

J Šimunović, J Gašperšič, U Černigoj… - Biotechnology and …, 2023 - Wiley Online Library
Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N‐
glycosylation sites carrying mainly complex type N‐glycans. Its glycosylation is altered in …

Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

U Andjelković, S Tufegdžić, M Popović - Electrophoresis, 2017 - Wiley Online Library
The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low
back pressure, easy preparation process and miniaturisation, and the availability of different …

Speciation of copper in human serum using conjoint liquid chromatography on short-bed monolithic disks with UV and post column ID-ICP-MS detection

K Marković, M Cemazar, G Sersa, R Milačič… - Journal of Analytical …, 2022 - pubs.rsc.org
Ceruloplasmin (Cp) is the major copper-carrying (Cu) protein in human plasma. Due to
copper's important physiological functions and its role in various diseases, there is a need to …

Liquid‐phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis …

Z El Rassi, C Puangpila - Electrophoresis, 2017 - Wiley Online Library
This review article is an update of our previous one by C. Puangpila, E. Mayadunne, and Z.
El Rassi, Electrophoresis 2015, 36, 238–252. Similarly to the previous article, this review …

Semi‐high‐throughput isolation and N‐glycan analysis of human fibrinogen using monolithic supports bearing monoclonal anti‐human fibrinogen antibodies

U Vidic, I Trbojević‐Akmačić, U Černigoj… - …, 2017 - Wiley Online Library
Fibrinogen (FIB) is a secretory glycoprotein synthesized by hepatocytes that has a key role
in blood clotting. Its glycosylation has not been studied in detail and little is known about the …

Quantitative proteome analysis of Merkel cell carcinoma cell lines using SILAC

U Kotowski, BM Erović, J Schnöll, V Stanek, S Janik… - Clinical Proteomics, 2019 - Springer
Background Merkel cell carcinoma (MCC) is an aggressive neuroendocrine tumour of the
skin with growing incidence. To better understand the biology of this malignant disease …

Serum proteomic pattern in female stress urinary incontinence

M Koch, W Umek, E Hanzal, T Mohr, S Seyfert… - …, 2018 - Wiley Online Library
Abstract The pathophysiology of Stress Urinary Incontinence (SUI) is poorly understood. The
aim of this study was to identify the serum proteomic profile in patients with SUI and to …