Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review

Z Yang, Z Zhang - Biotechnology advances, 2018 - Elsevier
Pichia pastoris has been recognized as one of the most industrially important hosts for
heterologous protein production. Despite its high protein productivity, the optimization of P …

[HTML][HTML] Modular engineering for microbial production of carotenoids

C Li, CA Swofford, AJ Sinskey - Metabolic Engineering Communications, 2020 - Elsevier
There is an increasing demand for carotenoids due to their applications in the food, flavor,
pharmaceutical and feed industries, however, the extraction and synthesis of these …

[图书][B] Recent advancement in white biotechnology through fungi

AN Yadav, S Singh, S Mishra, A Gupta - 2019 - Springer
White biotechnology, or industrial biotechnology, is drawing much attention as a solution to
produce energy, chemicals and other materials from renewable resources. White …

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins

H Raschmanová, A Weninger, Z Knejzlík… - Applied Microbiology …, 2021 - Springer
Folding and processing of proteins in the endoplasmic reticulum (ER) are major
impediments in the production and secretion of proteins from Pichia pastoris (Komagataella …

[图书][B] Biotechnology of lactic acid bacteria: novel applications. 2

F Mozzi, RR Raya, GM Vignolo, JC Love - 2015 - Wiley Online Library
Over the past 100 years, industrial biotechnology has grown into a multibillion dollar market,
which now has even begun to include large parts of chemical, material, and fuel production …

Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production

WC Liu, S Inwood, T Gong, A Sharma… - Critical Reviews in …, 2019 - Taylor & Francis
Pichia pastoris is extensively used to produce various heterologous proteins. Amounts of
biopharmaceutical drugs and industrial enzymes have been successfully produced by fed …

Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects

ZE Duman-Özdamar, B Binay - The protein journal, 2021 - Springer
Industrial enzymes have been widely preferred in various industries such as chemical
production, food & beverage, pharmaceutical, textile, cosmetics, etc. due to the …

Improved Production of Recombinant Carboxylesterase FumDM by Co-Expressing Molecular Chaperones in Pichia pastoris

L Jiang, X Guan, H Liu, X Chang, J Sun, C Sun, C Zhao - Toxins, 2023 - mdpi.com
Fumonisins (FBs) are mycotoxins that threaten public health and food safety worldwide.
Enzymatic degradation of Fumonisin B1 (FB1) through decarboxylation has attracted much …

Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

C Bustos, J Quezada, R Veas, C Altamirano… - Metabolites, 2022 - mdpi.com
Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly
important microorganism for recombinant protein production. This yeast species has gained …

Screening endogenous signal peptides and protein folding factors to promote the secretory expression of heterologous proteins in Pichia pastoris

G Duan, L Ding, D Wei, H Zhou, J Chu, S Zhang… - Journal of …, 2019 - Elsevier
Secretory expression is most often desired but usually hampered by limitations of signal
peptide processing and protein folding in the methylotrophic yeast Pichia pastoris. To …