Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

We report the rational engineering of a remarkably stable yellow fluorescent protein
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …

Fixing cells with paraformaldehyde (PFA) is an essential step in numerous biological
techniques as it is thought to preserve a snapshot of biomolecular transactions in living cells …

Diverse RNAs and RNA-binding proteins form phase-separated, membraneless granules in
cells under stress conditions. However, the role of the prevalent mRNA methylation, m6A …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Since the discovery of lysosomes more than 70 years ago, much has been learned about
the functions of these organelles. Lysosomes were regarded as exclusively degradative …

Dendritic spines, the postsynaptic compartments of excitatory neurotransmission, have
different shapes classified from 'stubby'to 'mushroom-like'. Whereas mushroom spines are …

Despite being the target of extensive research efforts due to the COVID-19 (coronavirus
disease 2019) pandemic, relatively little is known about the dynamics of severe acute …