Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy

M Famulok, JS Hartig, G Mayer - Chemical reviews, 2007 - ACS Publications
Aptamers are single-stranded nucleic acid molecules that possess properties comparable to
those of protein monoclonal antibodies, and thus are clear alternatives to long established …

Single-stranded DNA (ssDNA) production in DNA aptamer generation

C Marimuthu, TH Tang, J Tominaga, SC Tan… - Analyst, 2012 - pubs.rsc.org
The discovery that synthetic short chain nucleic acids are capable of selective binding to
biological targets has made them to be widely used as molecular recognition elements …

Selection of HBsAg-specific DNA aptamers based on carboxylated magnetic nanoparticles and their application in the rapid and simple detection of hepatitis B virus …

Z Xi, R Huang, Z Li, N He, T Wang, E Su… - ACS Applied Materials …, 2015 - ACS Publications
Aptamers are short single-stranded DNA or RNA oligonucleotides and can be selected from
synthetic combinatorial libraries in vitro. They have a high binding affinity and specificity for …

Application of aptamers in diagnostics, drug-delivery and imaging

C Chandola, S Kalme, MG Casteleijn, A Urtti… - Journal of …, 2016 - Springer
Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target
with high specificity and affinity. Although aptamers are analogous to antibodies for a wide …

Progress in selection and biomedical applications of aptamers

Z Xi, R Huang, Y Deng, N He - Journal of biomedical …, 2014 - ingentaconnect.com
Aptamers are short single-stranded DNA or RNA, which can be selected from random
combinatorial library by SELEX in vitro. The SELEX technology has been modified over the …

Aptamer-based technologies for parasite detection

NE Brosseau, I Vallée, A Mayer-Scholl, M Ndao… - Sensors, 2023 - mdpi.com
Centuries of scientific breakthroughs have brought us closer to understanding and
managing the spread of parasitic diseases. Despite ongoing technological advancements in …

A mathematical analysis of SELEX

HA Levine, M Nilsen-Hamilton - Computational biology and chemistry, 2007 - Elsevier
Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure by which
a mixture of nucleic acids that vary in sequence can be separated into pure components with …

Disease‐specific biomarker discovery by aptamers

H Ulrich, C Wrenger - Cytometry Part A, 2009 - Wiley Online Library
RNA and DNA aptamers developed by an in vitro selection process, Systematic Evolution of
Ligands by EXponential enrichment (SELEX), comprise a novel class of high‐affinity and …

Neglected zoonotic diseases: Advances in the development of cell-penetrating and antimicrobial peptides against leishmaniosis and Chagas disease

SM Robledo, S Pérez-Silanes, C Fernández-Rubio… - Pathogens, 2023 - mdpi.com
In 2020, the WHO established the road map for neglected tropical diseases 2021–2030,
which aims to control and eradicate 20 diseases, including leishmaniosis and Chagas …

The affinity concept in bioseparation: evolving paradigms and expanding range of applications

K Mondal, MN Gupta - Biomolecular engineering, 2006 - Elsevier
The meaning of the word affinity in the context of protein separation has undergone
evolutionary changes over the years. The exploitation of molecular recognition phenomenon …