This tutorial review describes the principles and practices of electron capture and transfer
dissociation (ECD/ETD or ExD) mass spectrometry (MS) employed for peptide and protein …

For about three decades, the chemistry of cation radicals derived from organic molecules
had received a major impetus from mass spectrometry. This was because cation radicals …

Synthetic application of aminoketyl radicals [R–C•(O–) NR′ R ″] formed by a direct
electron capture into the amide bond is limited. Herein, we demonstrate addition of …

Direct detection and quantification of protein/peptide palmitoylation by mass spectrometry
(MS) is a challenging task because of the tendency of palmitoyl loss during sample …

Structural characterization of proteins in the gas phase is becoming increasingly popular,
highlighting the need for a greater understanding of how proteins behave in the absence of …

Disulfide bonds formed by a pair of cysteine residues in the peptides' backbone represent a
certain problem for their sequencing by means of mass spectrometry. As a rule, in …

The application of ion pre-activation with 266 nm ultraviolet (UV) laser irradiation combined
with electron capture dissociation (ECD) is demonstrated to enhance top-down mass …

In recent years, a number of novel tandem mass spectrometry approaches utilizing radical‐
driven peptide gas‐phase fragmentation chemistry have been developed. These …

We investigate the mechanism of disulfide bond cleavage in gaseous peptide and protein
ions initiated by a covalently-attached regiospecific acetyl radical using mass spectrometry …

In the present work, four, well-studied, model peptides (eg, substance P, bradykinin,
angiotensin I and AT-Hook 3) were used to correlate structural information provided by ion …