Bioorthogonal chemistry is a set of methods using the chemistry of non-native functional
groups to explore and understand biology in living organisms. In this review, we summarize …

Breakthroughs in precision cell surface engineering tools are supporting the rapid
development of programmable living assemblies with valuable features for tackling complex …

Despite a range of covalent protein modifications, few techniques exist for quantification of
protein bioconjugation in cells. Here, we describe a novel method for quantifying in cellulo …

SNAP-tag is a single-turnover enzyme that has become a powerful tool, hence a popular
choice, of targeted cellular protein labeling. Three SNAP-tag substrates that carry the copper …

Current methods for the preparation of functional small-molecule fluorophores generally
require labor-intensive, multi-step synthetic routes for all the major chromophoric groups. In …

In classic bioorthogonal labeling experiments, the cell's biosynthetic machinery incorporates
bioorthogonal tags, creating tagged biomolecules that are subsequently reacted with a …

Current methods for the preparation of functional small-molecule fluorophores generally
require labor-intensive, multi-step synthetic routes for all the major chromophore groups. In …