Real-time PCR for mRNA quantitation

ML Wong, JF Medrano - Biotechniques, 2005 - Taylor & Francis
Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

Basic principles of real-time quantitative PCR

M Arya, IS Shergill, M Williamson… - Expert review of …, 2005 - Taylor & Francis
Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of
nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field …

[HTML][HTML] Reactive oxygen species amplify protein kinase C signaling in high glucose-induced fibronectin expression by human peritoneal mesothelial cells

HB Lee, MR Yu, JS Song, H Ha - Kidney international, 2004 - Elsevier
Reactive oxygen species amplify protein kinase C signaling in high glucose-induced
fibronectin expression by human peritoneal mesothelial cells. Background We previously …

Differential pattern of cytokine expression by macrophages infected in vitro with different Mycobacterium tuberculosis genotypes

R Chacon-Salinas, J Serafin-Lopez… - Clinical & …, 2005 - academic.oup.com
It has been shown recently that different genotypes of Mycobacterium tuberculosis induce
distinct immune responses in the host, as reflected by variations in cytokine and iNOS …

Role of zinc in cellular zinc trafficking and mineralization in a murine osteoblast-like cell line

M Nagata, B Lönnerdal - The Journal of nutritional biochemistry, 2011 - Elsevier
Zinc (Zn) supplementation stimulates bone growth in Zn-deficient humans and animals. A
biphasic pattern of mineralization has been observed in cultured osteoblasts; an initiation …

Subunit vaccine formulations based on recombinant envelope proteins of Chikungunya virus elicit balanced Th1/Th2 response and virus-neutralizing antibodies in …

M Khan, R Dhanwani, PVL Rao, M Parida - Virus research, 2012 - Elsevier
The recent resurgence of Chikungunya virus in India and Indian Ocean Islands with unusual
clinical severity is a matter of great public health concern. Despite the fact that CHIKV …

Angiotensin II mediates high glucose-induced TGF-β1 and fibronectin upregulation in HPMC through reactive oxygen species

H Noh, H Ha, MR Yu, YO Kim… - Peritoneal Dialysis …, 2005 - journals.sagepub.com
Objective To demonstrate the presence of an independent renin–angiotensin system (RAS)
in the peritoneum and to determine the role of locally produced angiotensin (Ang) II in high …

Real‐time polymerase chain reaction for the food microbiologist: technologies, applications, and limitations

SE Hanna, CJ Connor, HH Wang - Journal of Food Science, 2005 - Wiley Online Library
Rapid detection of pathogenic and spoilage microorganisms is essential for ensuring the
safety and quality of food. Real‐time polymerase chain reaction (PCR) technology has the …

CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

P Boeuf, I Vigan-Womas, D Jublot, S Loizon… - BMC immunology, 2005 - Springer
Background Real-time PCR is becoming a common tool for detecting and quantifying
expression profiling of selected genes. Cytokines mRNA quantification is widely used in …

The pro-opiomelanocortin genes in rainbow trout (Oncorhynchus mykiss): duplications, splice variants, and differential expression

EH Leder, JT Silverstein - Journal of endocrinology, 2006 - joe.bioscientifica.com
Pro-opiomelanocortin (POMC) is a precursor for several important peptide hormones
involved in a variety of functions ranging from stress response to energy homeostasis. In …