Synthetic biology tools to engineer microbial communities for biotechnology

NS McCarty, R Ledesma-Amaro - Trends in biotechnology, 2019 - cell.com
Microbial consortia have been used in biotechnology processes, including fermentation,
waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly …

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

SK Lee, H Chou, TS Ham, TS Lee… - Current opinion in …, 2008 - Elsevier
The ability to generate microorganisms that can produce biofuels similar to petroleum-based
transportation fuels would allow the use of existing engines and infrastructure and would …

Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors

AJ Meyer, TH Segall-Shapiro, E Glassey… - Nature chemical …, 2019 - nature.com
Cellular processes are carried out by many genes, and their study and optimization requires
multiple levers by which they can be independently controlled. The most common method is …

A fast, robust and tunable synthetic gene oscillator

J Stricker, S Cookson, MR Bennett, WH Mather… - Nature, 2008 - nature.com
One defining goal of synthetic biology is the development of engineering-based approaches
that enable the construction of gene-regulatory networks according to 'design specifications' …

BglBrick vectors and datasheets: a synthetic biology platform for gene expression

TS Lee, RA Krupa, F Zhang, M Hajimorad… - Journal of biological …, 2011 - Springer
Background As engineered biological systems become more complex, it is increasingly
common to express multiple operons from different plasmids and inducible expression …

Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids

L Fang, J Fan, S Luo, Y Chen, C Wang, Y Cao… - Nature …, 2021 - nature.com
To construct a superior microbial cell factory for chemical synthesis, a major challenge is to
fully exploit cellular potential by identifying and engineering beneficial gene targets in …

Synthetic biosensors for precise gene control and real-time monitoring of metabolites

JK Rogers, CD Guzman, ND Taylor… - Nucleic acids …, 2015 - academic.oup.com
Abstract Characterization and standardization of inducible transcriptional regulators has
transformed how scientists approach biology by allowing precise and tunable control of …

Next-generation synthetic gene networks

TK Lu, AS Khalil, JJ Collins - Nature biotechnology, 2009 - nature.com
Synthetic biology is focused on the rational construction of biological systems based on
engineering principles. During the field's first decade of development, significant progress …

AraC protein, regulation of the l-arabinose operon in Escherichia coli, and the light switch mechanism of AraC action

R Schleif - FEMS microbiology reviews, 2010 - academic.oup.com
This review covers the physiological aspects of regulation of the arabinose operon in
Escherichia coli and the physical and regulatory properties of the operon's controlling gene …

Synthetic biology for synthetic chemistry

JD Keasling - ACS chemical biology, 2008 - ACS Publications
The richness and versatility of biological systems make them ideally suited to solve some of
the world's most significant challenges, such as converting cheap, renewable resources into …