Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment

Y Cao, MR Raith, JF Griffith - water research, 2015 - Elsevier
Despite wide application to beach water monitoring and microbial source identification,
results produced by quantitative PCR (qPCR) methods are subject to bias introduced by …

Advancements in mitigating interference in quantitative polymerase chain reaction (qPCR) for microbial water quality monitoring

SP Nappier, A Ichida, K Jaglo, R Haugland… - Science of the Total …, 2019 - Elsevier
Abstract The United States Environmental Protection Agency's (EPA) 1 2012 Recreational
Water Quality Criteria included an Enterococcus spp. quantitative polymerase chain reaction …

[HTML][HTML] Monitoring urban beaches with qPCR vs. culture measures of fecal indicator bacteria: Implications for public notification

S Dorevitch, A Shrestha, S DeFlorio-Barker… - Environmental …, 2017 - Springer
Abstract Background The United States Environmental Protection Agency has established
methods for testing beach water using the rapid quantitative polymerase chain reaction …

[HTML][HTML] Evaluation of rapid qPCR method for quantification of E. coli at non-point source impacted Lake Michigan beaches

A Shrestha, S Dorevitch - Water research, 2019 - Elsevier
Abstract Most Great Lakes communities rely on culture-based E. coli methods for monitoring
fecal indicator bacteria (FIB) at recreational beaches. These cultivation methods require 18 …

[HTML][HTML] Rapid qPCR-based water quality monitoring in New York state recreational waters

CP Fernández-Baca, CM Spirito, JS Bae… - Frontiers in …, 2021 - frontiersin.org
Public swimming beaches often rely on culture-based methods to determine if fecal indicator
bacteria (FIB) levels are greater than health risk-based beach action values (BAV). The slow …

Absolute quantification of enterococcal 23S rRNA gene using digital PCR

D Wang, KM Yamahara, Y Cao… - Environmental Science & …, 2016 - ACS Publications
We evaluated the ability of chip-based digital PCR (dPCR) to quantify enterococci, the fecal
indicator recommended by the United States Environmental Protection Agency (USEPA) for …

Near real-time notification of water quality impairments in recreational freshwaters using rapid online detection of β-D-glucuronidase activity as a surrogate for …

M Cazals, R Stott, C Fleury, F Proulx, M Prévost… - Science of the total …, 2020 - Elsevier
Waterborne disease outbreaks associated with recreational waters continue to be reported
around the world despite existing microbiological water quality monitoring frameworks. Most …

Comparison of Five Detection and Quantification Methods for Phytophthora ramorum in Stream and Irrigation Water

L Rollins, K Coats, M Elliott, G Chastagner - Plant disease, 2016 - Am Phytopath Society
Propagules of Phytophthora ramorum, the causal agent of sudden oak death (SOD) and
ramorum blight, can be recovered from infested stream and nursery irrigation runoff using …

Marker genes of fecal indicator bacteria and potential pathogens in animal feces in subtropical catchments

W Ahmed, C O'Dea, N Masters, A Kuballa… - Science of the Total …, 2019 - Elsevier
We investigated the abundance of marker genes of two fecal indicator bacteria (FIB) and
eight potential pathogens in fecal samples of humans (n= 14) and 10 domestic and native …

The next-generation PCR-based quantification method for ambient waters: digital PCR

Y Cao, JF Griffith, SB Weisberg - Marine genomics: methods and protocols, 2016 - Springer
Real-time quantitative PCR (qPCR) is increasingly being used for ambient water monitoring,
but development of digital polymerase chain reaction (digital PCR) has the potential to …