The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve …

In higher eukaryotes, fine-tuned activation of protein-coding genes and many non-coding
RNAs pivots around the regulated activity of RNA polymerase II (Pol II). The Integrator …

Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

The dynamic regulation of transcription elongation by RNA polymerase II (Pol II) is an
integral part of the implementation of gene expression programmes during development. In …

Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …

Recent advances in sequencing techniques that measure nascent transcripts and that
reveal the positioning of RNA polymerase II (Pol II) have shown that the pausing of Pol II in …

We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant,
PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription …

The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …

Proteotoxic stress, that is, stress caused by protein misfolding and aggregation, triggers the
rapid and global reprogramming of transcription at genes and enhancers. Genome-wide …