Small molecule drug discovery has been propelled by the continual development of novel
scientific methodologies to occasion therapeutic advances. Although established …

Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …

Ion mobility spectrometry/mass spectrometry (IMS/MS) is widely used to study various levels
of protein structure. Here, we review the current state of affairs in tandem-trapped ion …

“Top-down” proteomics analyzes intact proteins and identifies proteoforms by their intact
mass as well as the observed fragmentation pattern in tandem mass spectrometry (MS/MS) …

Top-down proteomics provides a straightforward approach to the level of proteoforms but
remains technologically challenging. Using ion mobility spectrometry/mass spectrometry …

Rationale Tandem‐ion mobility spectrometry/mass spectrometry methods have recently
gained traction for the structural characterization of proteins and protein complexes …

Ultraviolet photodissociation is a fast, photon-mediated fragmentation method that yields
high sequence coverage and informative cleavages of biomolecules. In this work, 193 nm …

Top-down mass spectrometry (MS) is a versatile tool that has been employed to investigate
both protein sequence and structure. Although a variety of different fragmentation methods …

MALDI imaging allows for the near-cellular profiling of proteoforms directly from microbial,
plant, and mammalian samples. Despite detecting hundreds of proteoforms, identification of …

Protein glycosylation is implicated in a wide array of diseases, yet glycoprotein analysis
remains elusive owing to the extreme heterogeneity of glycans, including …