Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting

MK Purcell, RG Getchell, CA McClure… - Journal of Aquatic …, 2011 - academic.oup.com
Real‐time, or quantitative, polymerase chain reaction (qPCR) is quickly supplanting other
molecular methods for detecting the nucleic acids of human and other animal pathogens …

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future …

JV Samsonova, NY Saushkin, AP Osipov - Veterinary Research …, 2022 - Springer
Abstract Dried Blood Spots (DBS) technology has become a valuable tool in medical
studies, however, in veterinary and biological research DBS technology applications are still …

Low-cost and rapid method of DNA extraction from scaled fish blood and skin mucus

L Gui, X Li, S Lin, Y Zhao, P Lin, B Wang, R Tang… - Viruses, 2022 - mdpi.com
PCR-based DNA amplification has been one of the major methods in aquaculture research
for decades, although its use outside the modern laboratory environment is limited due to …

A simple filter paper-based method for transporting and storing Enterocytozoon hepatopenaei DNA from infected Litopenaeus vannamei tissues

K Karthikeyan, R Saranya, R Bharath, R Vidya… - Journal of Invertebrate …, 2020 - Elsevier
The microsporidian parasite Enterocytozoon hepatopenaei (EHP) causes a significant
negative impact in shrimp aquaculture. A diagnostic procedure for detecting EHP in shrimp …

The 2023 National Proficiency Test for molecular detection of shrimp infectious myonecrosis virus (IMNV) in China

C Li, B Yang, M Wang, X Wan, W Yu, W Li, X Song… - Aquaculture, 2025 - Elsevier
Infectious myonecrosis virus (IMNV), an emerging virus that can cause severe viral disease
in penaeid shrimp, was first confirmed to have spread to China in 2020. In order to …

Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus: Molecular cloning and characterization

T Mekata, S Okugawa, M Inada, M Yoshimine… - Molecular and Cellular …, 2011 - Elsevier
The scavenger receptor, Croquemort is a member of the CD36 superfamily comprising
transmembrane proteins involved in the recognition of polyanionic ligands. Various …

Double‐stranded RNA‐mediated silencing of the white spot syndrome virus VP28 gene in kuruma shrimp, Marsupenaeus japonicus

R Sudhakaran, T Mekata, T Kono, M Inada… - Aquaculture …, 2011 - Wiley Online Library
Recent applications of RNA interference technology against the white spot syndrome virus
(WSSV) were found to be promising and effective antiviral strategies. In the present study, a …

[PDF][PDF] FTA 卡保存对虾传染性皮下和造血组织坏死病毒DNA 洗脱方法的优化

连新宇, 王秀华, 李晨, 张庆利, 苟紫玥… - PROGRESS IN …, 2024 - aquaticjournal.com
摘要传染性皮下和造血组织坏死病毒(infectious hypodermal and haematopoietic necrosis
virus, IHHNV) 是危害虾类健康养殖的重要病原, 为寻找一种快捷保存及分离IHHNV DNA …

based archiving of biological samples from fish for detecting betanodavirus

A Navaneeth Krishnan, T Bhuvaneswari… - Archives of …, 2016 - Springer
This study was carried out to evaluate the efficiency of the Flinders Technology Associates
(FTA®) card (Whatman®) as a sampling device and storage platform for RNA from …

Deciphering the DNA repair protein, Rad23 from kuruma shrimp Marsupenaeus japonicus: full‐length cDNA cloning and characterization

R Sudhakaran, S Okugawa, T Mekata… - Letters in applied …, 2011 - academic.oup.com
Aims: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living
systems. NER process‐related host factors are believed to aid recovery steps during viral …