The past quarter century has witnessed rapid developments of fluorescence microscopy
techniques that enable structural and functional imaging of biological specimens at …

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

The extracellular space (ECS) of the brain has an extremely complex spatial organization,
which has defied conventional light microscopy. Consequently, despite a marked interest in …

Adaptive optics is becoming a valuable tool for high resolution microscopy, providing
correction for aberrations introduced by the refractive index structure of specimens. This is …

Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

As one of the most powerful tools in the biological investigation of cellular structures and
dynamic processes, fluorescence microscopy has undergone extraordinary developments in …

Understanding cellular organization demands the best possible spatial resolution in all three
dimensions. In fluorescence microscopy, this is achieved by 4Pi nanoscopy methods that …

Direct laser writing (DLW) enables arbitrary three-dimensional nanofabrication. However,
the diffraction limit poses a major obstacle for realizing nanometer-scale features …

STED microscopy is one of several fluorescence microscopy techniques that permit imaging
at higher spatial resolution than what the diffraction-limit of light dictates. STED imaging is …

Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D)
visualization of dynamic nanoscale structures in living cells, offering unique insights into …