The basic factor underlying the success of the tissue culture, large-scale micropropagation
and genetic transformation of any plant species is regeneration. This has been achieved …

Thin cell layer explants are thin strips of tissue composed of the epidermis plus 1-10 layers
of subepidermal parenchyma cells. This review describes the effects of endogenous and …

Shoots of orange jessamine (Murraya paniculata) a member of the Rutaceae family flowered
in vitro on half-strength MT basal medium containing 5% sucrose. The highest percentage …

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum
L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of …

In vitro culture techniques have done much to further our understanding of the physiology of
flower induction and evocation. Contributions in the literature are extensively reviewed, the …

Thin cell-layer explants (TCLs) have been proposed as favorable tissues for the study of
root, vegetative shoot and flower formation. We tested the effects of pH, light quality, light …

Nicotiana is one of the larger genera of the Solanaceae, presently comprising 66 species,
some of which are used commercially. The most widely used one is the common tobacco, N …

After many years of intensive research, which have not solved the mysteries of flowering,
there is a need for change and the use of new techniques in order to understand what is …

The capacity to form flower buds in thin-layer explants was studied in flowering plants of
several species, cultivars, and lines of Nicotiana differing in their response to photoperiod …

Floral determination in the terminal bud of the short-day plant Nicotiana tabacum cv.
Maryland Mammoth has been investigated. Plants grown continuously in short days …