Cofactor recycling is known to be crucial for amino acid synthesis. Hence, cofactor supply
was now analyzed for l‐valine to identify new targets for an improvement of production. The …

This article proposes a new concept termed ''yield analysis''(YA) as a method of extracting a
subset of elementary modes (EMs) essential for describing metabolic behaviors. YA can be …

Background An indirect approach is usually used to estimate the metabolic fluxes of an
organism: couple the available measurements with known biological constraints (eg …

Uniquely focusing on dynamic modeling, this volume incorporates metabolic regulation as a
survival mechanism for cells, by driving metabolism through optimal investment of its …

Background Microbial hosts offer a number of unique advantages when used as production
systems for both native and heterologous small-molecules. These advantages include high …

Abstract Motivation Elementary (flux) modes (EMs) have served as a valuable tool for
investigating structural and functional properties of metabolic networks. Identification of the …

Clostridium acetobutylicum is a strict anaerobe which exhibits two distinct steps in its
metabolic network. In the first step, sugars are oxidized to organic acids (acetic and butyric) …

Background Constraint-based models enable structured cellular representations in which
intracellular kinetics are circumvented. These models, combined with experimental data, are …

Motivation: Gene deletion and overexpression are critical technologies for designing or
improving the metabolic flux distribution of microbes. Some algorithms including flux …

Enzyme Control Flux (ECF) is a method of correlating enzyme activity and flux distribution.
The advantage of ECF is that the measurement integrates proteome data with metabolic flux …