The Escherichia coli replication origin oriC contains the initiator ATP-DnaA-Oligomerization
Region (DOR) and its flanking duplex unwinding element (DUE). In the Left-DOR subregion …

DNA replication is an essential process. Although the fundamental strategies to duplicate
chromosomes are similar in all free-living organisms, the enzymes of the three domains of …

Abstract In Escherichia coli, the DnaB helicase forms the basis for the assembly of the DNA
replication complex. The stability of DnaB at the replication fork is likely important for …

Unwinding of the replication origin and loading of DNA helicases underlie the initiation of
chromosomal replication. In Escherichia coli, the minimal origin oriC contains a duplex …

The DNA replication protein DnaA in Escherichia coli constructs higher-order complexes on
the origin, oriC, to unwind this region. DnaB helicase is loaded onto unwound oriC via …

The replicative helicase, DnaB, is a central component of the replisome and unwinds duplex
DNA coupled with immediate template-dependent DNA synthesis by the polymerase, Pol III …

Dedicated loader proteins play essential roles in bacterial DNA replication by opening ring-
shaped DnaB-family helicases and chaperoning single-stranded (ss) DNA into a central …

Assembly of bacterial ring-shaped hexameric replicative helicases on single-stranded (ss)
DNA requires specialized loading factors. However, mechanisms implemented by these …

UvrD is a multi-functional Escherichia coli helicase. It is widely involved in DNA repair,
including its DNA unwinding role during mismatch repair and RNAP removal role during …

Bacterial DNA replication is a potential novel antibiotic target in the fight against increasing
anti-microbial resistance. To fully explore those opportunities, a full understanding of the …