Molecular biology of cyanobacterial salt acclimation

M Hagemann - FEMS microbiology reviews, 2011 - academic.oup.com
High and changing salt concentrations represent major abiotic factors limiting the growth of
microorganisms. During their long evolution, cyanobacteria have adapted to aquatic habitats …

An insight into iTRAQ: where do we stand now?

C Evans, J Noirel, SY Ow, M Salim… - Analytical and …, 2012 - Springer
The iTRAQ (isobaric tags for relative and absolute quantification) technique is widely
employed in proteomic workflows requiring relative quantification. Here, we review the …

Technical, experimental, and biological variations in isobaric tags for relative and absolute quantitation (iTRAQ)

CS Gan, PK Chong, TK Pham… - Journal of proteome …, 2007 - ACS Publications
We assess the reliability of isobaric-tags for relative and absolute quantitation (iTRAQ),
based on different types of replicate analyses taking into account technical, experimental …

Cyanobacterial hydrogenases: diversity, regulation and applications

P Tamagnini, E Leitao, P Oliveira… - FEMS microbiology …, 2007 - academic.oup.com
Cyanobacteria may possess two distinct nickel-iron (NiFe)-hydrogenases: an uptake
enzyme found in N 2-fixing strains, and a bidirectional one present in both non-N 2-fixing …

Isobaric tags for relative and absolute quantitation (iTRAQ) reproducibility: Implication of multiple injections

PK Chong, CS Gan, TK Pham… - Journal of proteome …, 2006 - ACS Publications
We analyzed 10 isobaric tags for relative and absolute quantitation (iTRAQ) experiments
using three different model organisms across the domains of life: Saccharomyces cerevisiae …

Minimising iTRAQ ratio compression through understanding LC‐MS elution dependence and high‐resolution HILIC fractionation

SY Ow, M Salim, J Noirel, C Evans, PC Wright - Proteomics, 2011 - Wiley Online Library
Application of iTRAQ‐based workflows for protein profiling has become widespread.
Concomitantly, the idiosyncratic limitations of iTRAQ, such as its tendency to underestimate …

Proteome analysis of salt stress response in the cyanobacterium Synechocystis sp. strain PCC 6803

S Fulda, S Mikkat, F Huang, J Huckauf, K Marin… - …, 2006 - Wiley Online Library
In the present study, changes in protein synthesis patterns after salt shock visualized by 35S‐
methionine labeling and the changed protein composition in salt‐acclimated cells of the …

Mechanisms of Bacillus cereus biofilm formation: an investigation of the physicochemical characteristics of cell surfaces and extracellular proteins

E Karunakaran, CA Biggs - Applied microbiology and biotechnology, 2011 - Springer
Microbial biofilms contribute to biofouling in a wide range of processes from medical
implants to processed food. The extracellular polymeric substances (EPS) are implicated in …

Global proteomics reveal an atypical strategy for carbon/nitrogen assimilation by a cyanobacterium under diverse environmental perturbations

KM Wegener, AK Singh, JM Jacobs, T Elvitigala… - Molecular & Cellular …, 2010 - ASBMB
Cyanobacteria, the only prokaryotes capable of oxygenic photosynthesis, are present in
diverse ecological niches and play crucial roles in global carbon and nitrogen cycles. To …

Quantitative Shotgun Proteomics of Enriched Heterocysts from Nostoc sp. PCC 7120 Using 8-Plex Isobaric Peptide Tags

SY Ow, T Cardona, A Taton, A Magnuson… - Journal of proteome …, 2008 - ACS Publications
The filamentous cyanobacterium Nostoc sp. strain PCC 7120 is capable of fixing
atmospheric nitrogen. The labile nature of the core process requires the terminal …