Abstract Systems analysis of metabolic and growth functions in microbial organisms is
rapidly developing and maturing. Such studies are enabled by reconstruction, at the …

Metabolism is the complex network of chemical reactions occurring within every cell and
organism, maintaining life, mediating ecosystem processes and affecting Earth's climate …

Background Metabolomics has emerged as a powerful tool in the quantitative identification
of physiological and disease-induced biological states. Extracellular metabolome or …

Microorganisms produce and secrete many primary and secondary metabolites to the
surrounding environment during their growth. Therefore, extracellular metabolites provide …

Highlights•We now have metabolic network models; the metabolome is represented by their
nodes.•Metabolite levels are sensitive to changes in enzyme activities.•Drugs hitchhike on …

An increasing number of genome‐scale reconstructions of intracellular biochemical
networks are being generated. Coupled with these stoichiometric models, several systems …

Stoichiometric analysis of metabolic networks allows the calculation of possible metabolic
flux distributions in the absence of kinetic data. In order to predict which of the possible …

The exploitation of microorganisms in industrial, medical, food and environmental
biotechnology requires a comprehensive understanding of their physiology. The availability …

With the advent of modern omics technologies, it has become feasible to reconstruct (quasi-)
whole-cell metabolic networks and characterize them in more and more detail. Computer …

Highlights•Metabolic flux is a fundamental aspect of life.•Measuring and modeling metabolic
flux are challenging.•Boundary fluxes are the exchange rate of nutrients in and out of …