The Drosophila blastoderm embryo is a classic model for the study of the genetics of pattern
formation. In recent years, quantitative empirical approaches have been employed …

Summary Paused RNA polymerase (Pol II) is a pervasive feature of Drosophila embryos and
mammalian stem cells, but its role in development is uncertain. Here, we demonstrate that a …

Light-sheet microscopy is a powerful method for imaging the development and function of
complex biological systems at high spatiotemporal resolution and over long time scales …

Zygotic genome activation (ZGA) is a major genome programming event whereby the cells
of the embryo begin to adopt specified fates. Experiments in Drosophila and zebrafish have …

Dorsoventral pattern of Drosophila embryo is specified by the nuclear localization gradient
of the transcription factor Dorsal. Genetic and genomic studies of this morphogen gradient …

The greatest mystery of life is how a single fertilized egg develops into a fully functioning,
sometimes conscious multicellular organism. Embryogenesis Explained offers a new theory …

Here we describe a protocol for the fabrication and use of a microfluidic device to rapidly
orient> 700 Drosophila embryos in parallel for end-on imaging. The protocol describes …

Transient activation of the highly conserved extracellular-signal-regulated kinase (ERK)
establishes precise patterns of cell fates in developing tissues. Quantitative parameters of …

ERK controls gene expression in development, but mechanisms that link ERK activation to
changes in transcription are not well understood. We used high-resolution analysis of …

The early Drosophila embryo is patterned by graded distributions of maternal transcription
factors. Recent studies revealed that pattern formation by these graded signals depends on …