Quantifying how post-transcriptional noise and gene copy number variation bias transcriptional parameter inference from mRNA distributions
Transcriptional rates are often estimated by fitting the distribution of mature mRNA numbers
measured using smFISH (single molecule fluorescence in situ hybridization) with the …
measured using smFISH (single molecule fluorescence in situ hybridization) with the …
[HTML][HTML] Multimodal techniques and strategies for chemical and metabolic imaging at the single-cell level
A Kesharwani, V Gujrati - Current Opinion in Biotechnology, 2024 - Elsevier
Highlights•This review covers advanced SCI techniques enhancing sensitivity, resolution,
and data reproducibility.•We cover fluorescence, super resolution microscopy, optoacoustic …
and data reproducibility.•We cover fluorescence, super resolution microscopy, optoacoustic …
Quantifying and correcting bias in transcriptional parameter inference from single-cell data
R Grima, PM Esmenjaud - Biophysical Journal, 2024 - cell.com
The snapshot distribution of mRNA counts per cell can be measured using single-molecule
fluorescence in situ hybridization or single-cell RNA sequencing. These distributions are …
fluorescence in situ hybridization or single-cell RNA sequencing. These distributions are …
Transcriptional bursting dynamics in gene expression
Q Zhang, W Cao, J Wang, Y Yin, R Sun, Z Tian… - Frontiers in …, 2024 - frontiersin.org
Gene transcription is a stochastic process that occurs in all organisms. Transcriptional
bursting, a critical molecular dynamics mechanism, creates significant heterogeneity in …
bursting, a critical molecular dynamics mechanism, creates significant heterogeneity in …
[PDF][PDF] Systematic biases in transcriptional parameters inferred from single-cell snapshot data
R Grima, PM Esmenjaud - bioRxiv, 2023 - researchgate.net
The snapshot distribution of mRNA counts per cell can be readily measured using single
molecule FISH or single-cell RNA sequencing. These distributions are often fit to the steady …
molecule FISH or single-cell RNA sequencing. These distributions are often fit to the steady …
[HTML][HTML] Protocol for FRAP-based estimation of nuclear import and export rates in single yeast cells
Here, we present a protocol for estimating nuclear transport parameters in single cells. We
describe steps for performing four consecutive fluorescence recovery after photobleaching …
describe steps for performing four consecutive fluorescence recovery after photobleaching …
The nuclear-cytoplasmic ratio controls the cell cycle period in compartmentalized frog egg extract
Each proliferating cell replicates its DNA and internal components before distributing this
material evenly to its daughters. Although the regulation of cyclin-dependent kinases (Cdks) …
material evenly to its daughters. Although the regulation of cyclin-dependent kinases (Cdks) …