Microglial research has advanced considerably in recent decades yet has been constrained
by a rolling series of dichotomies such as" resting versus activated" and" M1 versus M2." …

The concept of an epigenetic landscape describing potential cellular fates arising from
pluripotent cells, first advanced by Conrad Waddington, has evolved in light of experiments …

Single-cell biology is facing a crisis of sorts. Vast numbers of single-cell molecular profiles
are being generated, clustered and annotated. However, this is overwhelmingly ad hoc, and …

Trajectory inference approaches analyze genome-wide omics data from thousands of single
cells and computationally infer the order of these cells along developmental trajectories …

A reflection of our ultimate understanding of a complex system is our ability to control its
behavior. Typically, control has multiple prerequisites: it requires an accurate map of the …

Life is characterized by a myriad of complex dynamic processes allowing organisms to grow,
reproduce, and evolve. Physical approaches for describing systems out of thermodynamic …

Recently, direct reprogramming between divergent lineages has been achieved by the
introduction of regulatory transcription factors. This approach may provide alternative cell …

Summary Single-cell (sc) RNA-seq, together with RNA velocity and metabolic labeling,
reveals cellular states and transitions at unprecedented resolution. Fully exploiting these …

Many systems on our planet shift abruptly and irreversibly from the desired state to an
undesired state when forced across a “tipping point”. Some examples are mass extinctions …

Forward and backward transitions between epithelial and mesenchymal phenotypes play
crucial roles in embryonic development and tissue repair. Aberrantly regulated transitions …