To preserve the integrity of their genome, bacteria rely on several genome maintenance
mechanisms that are co-ordinated with the cell cycle. All members of the Vibrio family have a …

Faithful transmission of the genome from one generation to the next is key to life in all
cellular organisms. In the majority of bacteria, the genome is comprised of a single circular …

An important step towards understanding the mechanistic basis of the central dogma is the
quantitative characterization of the dynamics of nucleic-acid-bound molecular motors in the …

Three types of structurally related structural maintenance of chromosomes (SMC)
complexes, referred to as condensins, have been identified in bacteria. Smc-ScpAB is …

The field of cell-free synthetic biology is an emerging branch of engineered biology that
allows for rapid prototyping of biological designs and, in its own right, is becoming a venue …

Vibrio cholerae, the causative agent of cholera epidemics, is a rod-shaped bacterium with a
highly polarized cellular organization. It can survive harmful growth conditions by entering a …

Replication is initiated bidirectionally in the three domains of life by the assembly of two
replication forks at an origin of replication. This is made possible by the recruitment of two …

Multipartite bacterial genomes pose challenges for genome engineering and the
establishment of additional replicons. We simplified the tripartite genome structure (3.65 …

DNA replication is essential for the proliferation of all cells. Bacterial chromosomes are
replicated bidirectionally from a single origin of replication, with replication proceeding at …

In Escherichia coli, DNA replication termination is orchestrated by two clusters of Ter sites
forming a DNA replication fork trap when bound by Tus proteins. The formation of a …