Co-transcriptional gene regulation in eukaryotes and prokaryotes
M Shine, J Gordon, L Schärfen, D Zigackova… - … Reviews Molecular Cell …, 2024 - nature.com
Many steps of RNA processing occur during transcription by RNA polymerases. Co-
transcriptional activities are deemed commonplace in prokaryotes, in which the lack of …
transcriptional activities are deemed commonplace in prokaryotes, in which the lack of …
[HTML][HTML] The role of mRNA quality control in the aging of Caenorhabditis elegans
HC Kwon, Y Bae, SJV Lee - Molecules and Cells, 2023 - Elsevier
The proper maintenance of mRNA quality that is regulated by diverse surveillance pathways
is essential for cellular homeostasis and is highly conserved among eukaryotes. Here, we …
is essential for cellular homeostasis and is highly conserved among eukaryotes. Here, we …
Aberrant splicing prediction across human tissues
Aberrant splicing is a major cause of genetic disorders but its direct detection in
transcriptomes is limited to clinically accessible tissues such as skin or body fluids. While …
transcriptomes is limited to clinically accessible tissues such as skin or body fluids. While …
Efficient, specific, and combinatorial control of endogenous exon splicing with dCasRx-RBM25
Efficient targeted control of splicing is a major goal of functional genomics and therapeutic
applications. Guide (g) RNA-directed, deactivated (d) Cas CRISPR enzymes fused to …
applications. Guide (g) RNA-directed, deactivated (d) Cas CRISPR enzymes fused to …
[HTML][HTML] Improved detection of aberrant splicing with FRASER 2.0 and the intron Jaccard index
Detection of aberrantly spliced genes is an important step in RNA-seq-based rare-disease
diagnostics. We recently developed FRASER, a denoising autoencoder-based method that …
diagnostics. We recently developed FRASER, a denoising autoencoder-based method that …
High-throughput sensitive screening of small molecule modulators of microexon alternative splicing using dual Nano and Firefly luciferase reporters
AJ Best, U Braunschweig, M Wu… - Nature …, 2024 - nature.com
Disruption of alternative splicing frequently causes or contributes to human diseases and
disorders. Consequently, there is a need for efficient and sensitive reporter assays capable …
disorders. Consequently, there is a need for efficient and sensitive reporter assays capable …
Structural insights into intron catalysis and dynamics during splicing
The group II intron ribonucleoprotein is an archetypal splicing system with numerous
mechanistic parallels to the spliceosome, including excision of lariat introns,. Despite the …
mechanistic parallels to the spliceosome, including excision of lariat introns,. Despite the …
Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion
JC Schmok, M Jain, LA Street, AT Tankka… - Nature …, 2024 - nature.com
RNA-binding proteins (RBPs) modulate alternative splicing outcomes to determine isoform
expression and cellular survival. To identify RBPs that directly drive alternative exon …
expression and cellular survival. To identify RBPs that directly drive alternative exon …
Systematic analysis of alternative exon-dependent interactome remodeling reveals multitasking functions of gene regulatory factors
Alternative splicing significantly expands biological complexity, particularly in the vertebrate
nervous system. Increasing evidence indicates that developmental and tissue-dependent …
nervous system. Increasing evidence indicates that developmental and tissue-dependent …
Splicing alterations in pancreatic ductal adenocarcinoma: a new molecular landscape with translational potential
E Alors-Pérez, S Pedraza-Arevalo… - Journal of Experimental …, 2023 - Springer
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal cancers
worldwide, mainly due to its late diagnosis and lack of effective therapies, translating into a …
worldwide, mainly due to its late diagnosis and lack of effective therapies, translating into a …