Many steps of RNA processing occur during transcription by RNA polymerases. Co-
transcriptional activities are deemed commonplace in prokaryotes, in which the lack of …

The proper maintenance of mRNA quality that is regulated by diverse surveillance pathways
is essential for cellular homeostasis and is highly conserved among eukaryotes. Here, we …

Aberrant splicing is a major cause of genetic disorders but its direct detection in
transcriptomes is limited to clinically accessible tissues such as skin or body fluids. While …

Efficient targeted control of splicing is a major goal of functional genomics and therapeutic
applications. Guide (g) RNA-directed, deactivated (d) Cas CRISPR enzymes fused to …

Detection of aberrantly spliced genes is an important step in RNA-seq-based rare-disease
diagnostics. We recently developed FRASER, a denoising autoencoder-based method that …

Disruption of alternative splicing frequently causes or contributes to human diseases and
disorders. Consequently, there is a need for efficient and sensitive reporter assays capable …

The group II intron ribonucleoprotein is an archetypal splicing system with numerous
mechanistic parallels to the spliceosome, including excision of lariat introns,. Despite the …

RNA-binding proteins (RBPs) modulate alternative splicing outcomes to determine isoform
expression and cellular survival. To identify RBPs that directly drive alternative exon …

Alternative splicing significantly expands biological complexity, particularly in the vertebrate
nervous system. Increasing evidence indicates that developmental and tissue-dependent …

Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal cancers
worldwide, mainly due to its late diagnosis and lack of effective therapies, translating into a …