Controlling and enhancing CRISPR systems
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
Inhibition of RNA-binding proteins with small molecules
P Wu - Nature Reviews Chemistry, 2020 - nature.com
Protein–RNA interactions have crucial roles in various cellular activities, which, when
dysregulated, can lead to a range of human diseases. The identification of small molecules …
dysregulated, can lead to a range of human diseases. The identification of small molecules …
Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems
Clustered regularly interspaced short palindromic repeats (CRISPR) together with their
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic
elements (MGEs). In response, some MGEs have developed strategies to bypass immunity …
elements (MGEs). In response, some MGEs have developed strategies to bypass immunity …
Machine-learning approach expands the repertoire of anti-CRISPR protein families
The CRISPR-Cas are adaptive bacterial and archaeal immunity systems that have been
harnessed for the development of powerful genome editing and engineering tools. In the …
harnessed for the development of powerful genome editing and engineering tools. In the …
Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer
C Mahendra, KA Christie, BA Osuna… - Nature …, 2020 - nature.com
CRISPR–Cas adaptive immune systems protect bacteria and archaea against their invading
genetic parasites, including bacteriophages/viruses and plasmids. In response to this …
genetic parasites, including bacteriophages/viruses and plasmids. In response to this …
AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation
W Sun, Z Cheng, J Wang, J Yang, X Li… - Proceedings of the …, 2023 - National Acad Sciences
Anti-CRISPR (Acr) proteins are encoded by phages and other mobile genetic elements and
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
Branched DNA switchable CRISPR-Cas12a system for sensing FEN1 activity
X Li, D Zhang, X Cai, X Shu, Z Zeng, S Ding… - Chemical Engineering …, 2023 - Elsevier
FEN1 plays a crucial role in tumor progression and proliferation, and it has been considered
a prospective cancer biomarker and druggable target. However, its detection is …
a prospective cancer biomarker and druggable target. However, its detection is …
Structures and strategies of anti-CRISPR-mediated immune suppression
T Wiegand, S Karambelkar… - Annual review of …, 2020 - annualreviews.org
More than 50 protein families have been identified that inhibit CRISPR (clustered regularly
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …