[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
SW Wang, C Gao, YM Zheng, L Yi, JC Lu, XY Huang… - Molecular cancer, 2022 - Springer
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …
Small-molecule inhibition of METTL3 as a strategy against myeloid leukaemia
E Yankova, W Blackaby, M Albertella, J Rak… - Nature, 2021 - nature.com
Abstract N 6-methyladenosine (m6A) is an abundant internal RNA modification, that is
catalysed predominantly by the METTL3–METTL14 methyltransferase complex,. The m6A …
catalysed predominantly by the METTL3–METTL14 methyltransferase complex,. The m6A …
METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation
The emerging" epitranscriptomics" field is providing insights into the biological and
pathological roles of different RNA modifications. The RNA methyltransferase METTL1 …
pathological roles of different RNA modifications. The RNA methyltransferase METTL1 …
Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects
H Li, Y Yang, W Hong, M Huang, M Wu… - Signal transduction and …, 2020 - nature.com
Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …
technologies has opened up the possibility of directly targeting and modifying genomic …
Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens
Functional genomics approaches can overcome limitations—such as the lack of
identification of robust targets and poor clinical efficacy—that hamper cancer drug …
identification of robust targets and poor clinical efficacy—that hamper cancer drug …
Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects
CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …
number of biases and artifacts that can confound the results. Here, we introduce Chronos …
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens
All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …
damage response (DDR) processes. Owing to their relevance to human disease, most …
Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control
Abstract N 6-methyladenosine (m6A) is an abundant internal RNA modification in both
coding and non-coding RNAs, that is catalysed by the METTL3–METTL14 methyltransferase …
coding and non-coding RNAs, that is catalysed by the METTL3–METTL14 methyltransferase …