[HTML][HTML] High-content CRISPR screening

C Bock, P Datlinger, F Chardon, MA Coelho… - Nature Reviews …, 2022 - nature.com
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

SW Wang, C Gao, YM Zheng, L Yi, JC Lu, XY Huang… - Molecular cancer, 2022 - Springer
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …

Small-molecule inhibition of METTL3 as a strategy against myeloid leukaemia

E Yankova, W Blackaby, M Albertella, J Rak… - Nature, 2021 - nature.com
Abstract N 6-methyladenosine (m6A) is an abundant internal RNA modification, that is
catalysed predominantly by the METTL3–METTL14 methyltransferase complex,. The m6A …

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation

EA Orellana, Q Liu, E Yankova, M Pirouz… - Molecular cell, 2021 - cell.com
The emerging" epitranscriptomics" field is providing insights into the biological and
pathological roles of different RNA modifications. The RNA methyltransferase METTL1 …

Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects

H Li, Y Yang, W Hong, M Huang, M Wu… - Signal transduction and …, 2020 - nature.com
Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens

FM Behan, F Iorio, G Picco, E Gonçalves, CM Beaver… - Nature, 2019 - nature.com
Functional genomics approaches can overcome limitations—such as the lack of
identification of robust targets and poor clinical efficacy—that hamper cancer drug …

Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects

JM Dempster, I Boyle, F Vazquez, DE Root, JS Boehm… - Genome biology, 2021 - Springer
CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

KR Sanson, RE Hanna, M Hegde, KF Donovan… - Nature …, 2018 - nature.com
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens

SW Awwad, A Serrano-Benitez, JC Thomas… - … Reviews Molecular Cell …, 2023 - nature.com
All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …

Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control

I Barbieri, K Tzelepis, L Pandolfini, J Shi… - Nature, 2017 - nature.com
Abstract N 6-methyladenosine (m6A) is an abundant internal RNA modification in both
coding and non-coding RNAs, that is catalysed by the METTL3–METTL14 methyltransferase …