The secretory pathway is responsible for the synthesis, folding, and delivery of a diverse
array of cellular proteins. Secretory protein synthesis begins in the endoplasmic reticulum …

Steady increase in the incidence of atherosclerosis is becoming a major concern not only in
the United States but also in other countries. One of the major risk factors for the …

In vitro synthesis of endoplasmic reticulum-derived transpofl vesicles has been reconstituted
with washed membranes and three soluble proteins (Sarl p, Secl3p complex, and Sec23p …

COPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and
Sec23/24p. PI 4-phosphate or PI 4, 5-bisphosphate is required for the binding of these …

IN yeast a type II integral membrane glycoprotein that is essential for transport vesicle
budding from the endoplasmic reticulum (ER) is encoded by SEC12 (refs 1–3). SAR1 was …

The trafficking of proteins within eukaryotic cells is achieved by the capture of cargo and
targeting molecules into vesicles that bud from a donor membrane and deliver their contents …

▪ Abstract Green fluorescent protein chimerae acting as reporters for protein localization and
trafficking within the secretory membrane system of living cells have been used in a wide …

Protein secretion is an essential process for living organisms. In eukaryotes, this
encompasses numerous steps mediated by several hundred cellular proteins. The core …

The binding and hydrolysis of guanosine triphosphate (GTP) by the small GTP-binding
protein Sar1p is required to form transport vesicles from the endoplasmic reticulum (ER) in …

Using three different trans dominant mutants of bovine ARF1 affecting GDP exchange or
GTP hydrolysis we demonstrate the central role of ARF1 in controlling vesicular traffic from …