Three-photon microscopy has been increasingly adopted for probing neural activities
beyond the typical two-photon imaging depth. In this review, we outline the unique …

After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …

Multiphoton fluorescence microscopy is a powerful technique for deep-tissue observation of
living cells. In particular, three-photon microscopy is highly beneficial for deep-tissue …

Visualization of the brain in its native environment is important for understanding common
brain diseases. Herein, bright luminogens with remarkable aggregation‐induced emission …

For a long time, optical imaging of the deep brain with high resolution has been a challenge.
Recently, with the advance in second near‐infrared (NIR‐II) bioimaging techniques and …

Multiphoton fluorescence microscopy (MPFM) has been a game-changer for optical
imaging, particularly for studying biological tissues deep within living organisms. MPFM …

Three-photon excitation has recently been demonstrated as an effective method to perform
intravital microscopy in deep, previously inaccessible regions of the mouse brain. The …

Second-order photonic topological insulators (SPTIs) with topologically protected corner
states possess extraordinary abilities of robust light steering in lower dimensions. However …

Behaviors emerge from activity throughout the brain, but noninvasive optical access in adult
vertebrate brains is limited. We show that three-photon (3P) imaging through the head of …

Three-photon fluorescence microscopic (3PFM) bioimaging is a promising imaging
technique for visualizing the brain in its native environment thanks to its advantages of high …